Transcriptomics,Genomics

Dataset Information

43

Role of Ezh2 on Skeletal Development


ABSTRACT: To study the role of Ezh2 in growth plate, we generated Ezh1 complete knockout, Ezh2 cartilage-specific knockout mice (Ezh1-/-, Col2-cre Ezh2 f/f) and compared it with Ezh2 wildtype littermates (Ezh1 -/-, Col2-cre Ezh2 +/+). Proliferative zone (PZ) and Hypertrophic zones (HZ) of proximal tibial growth plate were dissected from 3-day old mice by laser capture microdissection and RNA were isolated by pico pure RNA isolation kit Overall design: Total 24 samples, 6 WT PZ vs 6 KO PZ; 6 WT HZ vs 6 KO HZ, to identify genes up- or down-regulated upon deletion of Ezh2 expression in the growth plate

INSTRUMENT(S): [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]

SUBMITTER: Julian CK Lui  

PROVIDER: GSE84198 | GEO | 2016-11-09

SECONDARY ACCESSION(S): PRJNA328269

REPOSITORIES: GEO

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Publications

EZH1 and EZH2 promote skeletal growth by repressing inhibitors of chondrocyte proliferation and hypertrophy.

Lui Julian C JC   Garrison Presley P   Nguyen Quang Q   Ad Michal M   Keembiyehetty Chithra C   Chen Weiping W   Jee Youn Hee YH   Landman Ellie E   Nilsson Ola O   Barnes Kevin M KM   Baron Jeffrey J  

Nature communications 20161129


Histone methyltransferases EZH1 and EZH2 catalyse the trimethylation of histone H3 at lysine 27 (H3K27), which serves as an epigenetic signal for chromatin condensation and transcriptional repression. Genome-wide associated studies have implicated EZH2 in the control of height and mutations in EZH2 cause Weaver syndrome, which includes skeletal overgrowth. Here we show that the combined loss of Ezh1 and Ezh2 in chondrocytes severely impairs skeletal growth in mice. Both of the principal processe  ...[more]

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