Dataset Information


MEF-CM and UM_GTA re-stimulation experiment with human ES cells

ABSTRACT: Human embryonic stem cells can be maintained in a basic Serum Replacement (Invitrogen) based medium that has been conditioned on mouse embryonic fibroblasts (MEFs), yielding MEF-CM. Ligands secreted into the medium by the MEFs include Activin A, TGFß1, and Gremlin. This experiment served the purpose of identifying the short-term effects of MEF-CM and its substitute UM_GTA (unconditioned medium plus Activin A, TGFb1, and Gremlin) on gene expression in human embryonic stem cells. Keywords: Media / growth factor stimulation experiment Overall design: hES cells were grown in MEF-CM, then in unconditioned medium for 1 day. Cells were then re-stimulated with MEF-CM or UM_GTA for 4h to be compared to non-stimulated samples. The experiment was carried out in the presence of 5+5 (MEF-CM) or 10 ng/ml (UM, UM_GTA) of FGF2. Activin A was used at 20 ng/ml, TGFß1 at 2 ng/ml, and Gremlin at 200 ng/ml. Two biological replicates were hybridised per sample type.

INSTRUMENT(S): Sentrix HumanRef-8 Expression BeadChip

SUBMITTER: Boris Greber  

PROVIDER: GSE9059 | GEO | 2008-03-12



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Control of early fate decisions in human ES cells by distinct states of TGFbeta pathway activity.

Greber Boris B   Lehrach Hans H   Adjaye James J  

Stem cells and development 20081201 6

The mechanisms controlling self-renewal versus lineage commitment in human embryonic stem (hES) cells are not well understood. Nonetheless, current knowledge suggests a crucial role for TGFbeta signaling in controlling these early fate decisions. We have investigated the effects of TGFbeta pathway activation and inhibition on gene expression in hES cells. Our data reveal that SMAD 2/3 signaling directly supports NANOG expression, while SMAD 1/5/8 activation moderately represses SOX2. In addition  ...[more]

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