Dataset Information


Yeast linker histone HHO1p effect on transcription before and after heat shock

ABSTRACT: DNA array expression analysis comparing RNA transcripts in wild type and hho1 strains before and after a heat-shock from 25 to 37 degrees. Keywords: expression, heat shock Overall design: We used a reference design to hybridize three microarrays with the following cRNA samples labeled with Cy3 and Cy5, respectively: 1) wt25 and wt37, 2) mu37 and wt25, 3) mu25 and wt25. wt25, wt37, mu37 and mu25 represent cRNA made on RNA templates isolated from wild type cells grown at 25 degrees C, wild type cells following heat shock at 37 degrees C, hho1 delta cells following heat shock at 37 degrees C and hho1 delta cells grown at 25 degrees C, respectively.

INSTRUMENT(S): Agilent-013384 Yeast Oligo Microarray (V2) G4140B (Feature Number version)

SUBMITTER: Don Jay Katcoff  

PROVIDER: GSE9095 | GEO | 2008-07-01



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Yeast linker histone Hho1p is required for efficient RNA polymerase I processivity and transcriptional silencing at the ribosomal DNA.

Levy Anat A   Eyal Miri M   Hershkovits Gitit G   Salmon-Divon Mali M   Klutstein Michael M   Katcoff Don Jay DJ  

Proceedings of the National Academy of Sciences of the United States of America 20080807 33

Nucleosome core particles in eukaryotes are linked by a stretch of DNA that is usually associated with a linker histone. Here, we show in yeast, that the presence of yeast linker histone Hho1p represses expression of a pol II transcribed gene (MET15) embedded in the rDNA. In vivo deletions of Hho1p sequences showed that the second globular domain is sufficient for that repression, whereas the presence of the N terminus is required for its derepression. In contrast, a run-on assay confirmed by a  ...[more]

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