Transcriptomics,Genomics

Dataset Information

158

Multicellular Transcriptional Analysis of Mammalian Heart Regeneration


ABSTRACT: The inability of the adult mammalian heart to regenerate following injury represents a major barrier in cardiovascular medicine. In contrast, the neonatal mammalian heart retains a transient capacity for regeneration, which is lost shortly after birth. Defining the molecular mechanisms that govern regenerative capacity in the neonatal period remains a central goal in cardiac biology. Here, we construct a transcriptional atlas of multiple cardiac cell populations, which enables comparative analyses of the regenerative (neonatal) versus non-regenerative (adult) state for the first time. This work provides a comprehensive transcriptional resource of multiple cardiac cell populations during cardiac development, repair and regeneration. Our findings define a transcriptional program underpinning the neonatal regenerative state and identifies an epigenetic barrier to re-induction of the regenerative program in adult cardiomyocytes. Overall design: Cardiomyocytes, fibroblasts, leukocytes and endothelial cells from infarcted and non-infarcted neonatal (P1) and adult (P56) hearts were isolated by enzymatic dissociation and FACS. RNA sequencing (RNA-seq) was performed on these cell populations to generate a transcriptomic atlas of the major cardiac cell populations during cardiac development, repair and regeneration. In addition, we surveyed the epigenetic landscape of cardiomyocytes during post-natal maturation by performing deep sequencing of accessible chromatin regions using the Assay for Transposase-Accessible Chromatin (ATAC-seq) from purified cardiomyocyte nuclei (P1, P14 and P56).

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: James Hudson  

PROVIDER: GSE95755 | GEO | 2017-07-24

SECONDARY ACCESSION(S): PRJNA378265

REPOSITORIES: GEO

altmetric image

Publications


BACKGROUND:The inability of the adult mammalian heart to regenerate following injury represents a major barrier in cardiovascular medicine. In contrast, the neonatal mammalian heart retains a transient capacity for regeneration, which is lost shortly after birth. Defining the molecular mechanisms that govern regenerative capacity in the neonatal period remains a central goal in cardiac biology. Here, we assemble a transcriptomic framework of multiple cardiac cell populations during postnatal dev  ...[more]

Similar Datasets

| GSE95763 | GEO
| GSE95762 | GEO
2014-01-31 | E-GEOD-54530 | ArrayExpress
2018-02-02 | E-MTAB-6272 | ArrayExpress
2014-05-20 | E-GEOD-50531 | ArrayExpress
2017-03-22 | E-MTAB-5531 | ArrayExpress
2016-02-18 | E-GEOD-51013 | ArrayExpress
2016-02-15 | E-GEOD-51012 | ArrayExpress
2016-02-18 | E-GEOD-51018 | ArrayExpress
2016-02-18 | E-GEOD-51019 | ArrayExpress