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Tandem mass tag (TMT)-based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae

ABSTRACT: In this study, quantitative proteomics tandem mass tag (TMT) labeling technology was used to detect A Halolimnae protein was identified and quantitatively analyzed. Compared with the control group, 127521 and 672 differentially expressed proteins (DEPs) (Fold change ≤ 0.667 or ≥ 1.5, p<0.05) were identified under the treatment of 8%, 12% and 16% NaCl in the experimental group. GO (Gene Ontology) annotation analysis, COG (Clusters of Orthologous Groups of proteins) functional annotation and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway annotation were used for enrichment analysis of differentially expressed proteins. A Halolimnae adopted different strategies to deal with osmotic stress. The main mechanism of salt adaptation is to synthesize tetrahydropyrimidine and hydroxytetrahydropyrimidine, synthesize and absorb glycine betaine and proline, and accumulate different kinds and concentrations of compatible solutes in the cell. Among them, tetrahydropyrimidine synthesis-related proteins were up-regulated under low salt, high salt and extremely high salt conditions, and the contents of EctA, EctB, EctC and intracellular tetrahydropyrimidine increased significantly with the increase of salinity. Proteins related to the synthesis and transport of compatible solutes such as hydroxytetrahydropyrimidine, glycine betaine, proline, glutamine and glutamic acid were also up-regulated under different salinity conditions. At high salt concentration, cell metabolism slows down, but amino acid metabolism, carbohydrate metabolism and membrane transport related to salt adaptation are significantly regulated, providing energy and precursors for the accumulation of compatible solutes, and maintaining the balance of intracellular and extracellular permeability. In addition, the protein associated with Na+/K+ion transport seems to be in A Halolimnae played little role in the adaptation process of long-term osmotic stress.

ORGANISM(S): Alkalicoccus Halolimnae

SUBMITTER: Baisuo Zhao

PROVIDER: PXD039742 | iProX | Mon Jan 30 00:00:00 GMT 2023

REPOSITORIES: iProX

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Project description:Background: Salinity is an important abiotic stress that influences the physiological and metabolic activity, reproduction, growth and development of marine fish. It has been suggested that half-smooth tongue sole (Cynoglossus semilaevis), a euryhaline fish species, use a large amount of energy to maintain osmotic pressure balance when exposed to fluctuations in salinity. To delineate the molecular response of C. semilaevis to different levels of salinity, we performed RNA-seq analysis of the liver to identify the genes and molecular and biological processes involved in responding to salinity change. Results: The present study yielded 330.4 million clean reads, of which 83.9% were successfully mapped to the reference genome of C. semilaevis. One hundred twenty-eight differentially expressed genes (DEGs), including 43 up-regulated genes and 85 down-regulated genes, were identified. These DEGs were highly represented in metabolic pathways, steroid biosynthesis, terpenoid backbone biosynthesis, butanoate metabolism, glycerolipid metabolism and the 2-oxocarboxylic acid metabolism pathway. In addition, genes involved in metabolism, osmoregulation and ion transport, signal transduction, immune response and stress response, cytoskeleton remodeling, and apoptosis were affected during acclimation to low salinity. Genes acat2, fdps, hmgcr, hmgcs1, mvk, pmvk, ebp, lss, dhcr7, and dhcr24 were up-regulated and abat, ddc, acy1 were down-regulated in metabolic pathways. Genes aqp10 and slc6a6 were down-regulated in osmoregulation and ion transport. Genes abat, fdps, hmgcs1, mvk, pmvk and dhcr7 were first reported to be associated with salinity adaptation in teleosts. Conclusions: Our results revealed that metabolic pathways, especially lipid metabolism were important for salinity adaptation. The candidate genes identified from this study provide a basis for further studies to investigate the molecular mechanism of salinity adaptation and transcriptional plasticity in marine fish.

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Tandem mass tag (TMT)-based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae

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