Dataset Information

Tandem mass tag (TMT)-based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae

ABSTRACT: In this study, quantitative proteomics tandem mass tag (TMT) labeling technology was used to detect A Halolimnae protein was identified and quantitatively analyzed. Compared with the control group, 127521 and 672 differentially expressed proteins (DEPs) (Fold change ≤ 0.667 or ≥ 1.5, p<0.05) were identified under the treatment of 8%, 12% and 16% NaCl in the experimental group. GO (Gene Ontology) annotation analysis, COG (Clusters of Orthologous Groups of proteins) functional annotation and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway annotation were used for enrichment analysis of differentially expressed proteins. A Halolimnae adopted different strategies to deal with osmotic stress. The main mechanism of salt adaptation is to synthesize tetrahydropyrimidine and hydroxytetrahydropyrimidine, synthesize and absorb glycine betaine and proline, and accumulate different kinds and concentrations of compatible solutes in the cell. Among them, tetrahydropyrimidine synthesis-related proteins were up-regulated under low salt, high salt and extremely high salt conditions, and the contents of EctA, EctB, EctC and intracellular tetrahydropyrimidine increased significantly with the increase of salinity. Proteins related to the synthesis and transport of compatible solutes such as hydroxytetrahydropyrimidine, glycine betaine, proline, glutamine and glutamic acid were also up-regulated under different salinity conditions. At high salt concentration, cell metabolism slows down, but amino acid metabolism, carbohydrate metabolism and membrane transport related to salt adaptation are significantly regulated, providing energy and precursors for the accumulation of compatible solutes, and maintaining the balance of intracellular and extracellular permeability. In addition, the protein associated with Na+/K+ion transport seems to be in A Halolimnae played little role in the adaptation process of long-term osmotic stress.

ORGANISM(S): Alkalicoccus Halolimnae

SUBMITTER: Baisuo Zhao

PROVIDER: PXD039742 | iProX | Mon Jan 30 00:00:00 GMT 2023

REPOSITORIES: iProX

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Tandem mass tag-based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae BZ-SZ-XJ29T , a halophilic bacterium with a broad salinity range for optimal growth.

Xing Qinghua Q Mesbah Noha M NM Wang Haisheng H Zhang Yingjie Y Li Jun J Zhao Baisuo B

Environmental microbiology 20230604 10

The moderate halophilic bacterium Alkalicoccus halolimnae BZ-SZ-XJ29T exhibits optimum growth over a wide range of NaCl concentrations (8.3-12.3%, w/v; 1.42-2.1 mol L-1 ). However, its adaptive mechanisms to cope with high salt-induced osmotic stress remain unclear. Using TMT-based quantitative proteomics, the cellular proteome was assessed under low (4% NaCl, 0.68 mol L-1 NaCl, control (CK) group), moderate (8% NaCl, 1.37 mol L-1 NaCl), high (12% NaCl ...[more]

PMID: 37271582

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Dataset Information

Tandem mass tag (TMT)-based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae

Publications

Tandem mass tag-based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae BZ-SZ-XJ29<sup>T</sup> , a halophilic bacterium with a broad salinity range for optimal growth.

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