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The outer membrane protein of Riemerella anatipestifer YM strain interacting with duck vitronectin was screened by pull-down assay sequencing by mass spectrometry and search for complement escape related protein of Riemerella anatipestifer YM strain.

ABSTRACT: Riemerella anatipestifer can cause infectious serositis in ducklings with high mortality. Vitronectin acts as a complement regulator and plays a role in the terminal pathway of complement activation. It is a widely used strategy for Gram-negative bacteria to capture vitronectin to the surface of bacteria to inhibit complement activity. Therefore, we used duck vitronectin as bait protein, and screened the outer membrane protein of R. anatipestifer which can interact with duck vitronectin by pull-down assay, looking for the complement escape protein of R. anatipestifer, and laying a foundation for the prevention of R. anatipestifer.

ORGANISM(S): Riemerella Anatipestifer Ra-ym

SUBMITTER: Zutao Zhou

PROVIDER: PXD039753 | iProX | Wed Feb 01 00:00:00 GMT 2023

REPOSITORIES: iProX

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Outer membrane protein OMP76 of Riemerella anatipestifer contributes to complement evasion and virulence by binding to duck complement factor vitronectin.

Li Sen S Wang Yanhua Y Yang Rongkun R Zhu Xiaotong X Bai Hongying H Deng Xiaojian X Bai Jiao J Zhang Yang Y Xiao Yuncai Y Li Zili Z Liu Zhengfei Z Zhou Zutao Z

Virulence 20231201 1

Riemerella anatipestifer is an important bacterial pathogen in poultry. Pathogenic bacteria recruit host complement factors to resist the bactericidal effect of serum complement. Vitronectin (Vn) is a complementary regulatory protein that inhibits the formation of the membrane attack complex (MAC). Microbes use outer membrane proteins (OMPs) to hijack Vn for complement evasion. However, the mechanism by which R. anatipestifer achieves evasion is unclear. This study aimed to charact ...[more]

PMID: 37326479

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