Rose petal whole protein and proteomics of phosphorylation and ubiquitination modification
Ontology highlight
ABSTRACT: The 4D label-free quantitative proteomics (4D-LFQ) was used for quantification of proteome changes. Enrichment of phosphorylated peptides using immobilized metal ion affinity chromatography (IMAC) and enrichment of ubiquitinated peptides using anti-diglycine remnant (anti-K-ε-GG) antibodywere performed in phosphoproteome and ubiquitylome, respectively. Phosphorylation and ubiquitination quantifications were normalized by protein quantifications to eliminate the interference caused by protein abundance differences.
ORGANISM(S): Rosa Chinensis
SUBMITTER:
Yulin Cheng
PROVIDER: PXD040139 | iProX | Wed Feb 15 00:00:00 GMT 2023
REPOSITORIES: iProX
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