Dataset Information


1/4 MIC Contezolid, 1/4 MIC Linezolid and DMSO control group of Staphylococcus aureus biofilm quantitative proteomics comparative study to observe the global expression of protein Contezolid, Linezolid treatment groups and DMSO control group

ABSTRACT: S. aureus isolate in stable phase were inoculated into 15 ml TSBG plate culture, which contained 1/4 MIC of contezolid and linezolid treatment (DMSO as control) with 3 technical replicates in each group,. Incubate at 37 ° for 16 h and centrifuge at 5,000 rpm for 5 min to collect bacteria, including the formed biofilm bacteria. Wash with 1 ml PBS in water twice, transferred to a 1.5 ml EP tube, centrifuge and discard the supernatant. Lyse the cells by bead-beating at 4 °C in RIPA lysis buffer (1% Triton X-100, 1% deoxycholate, 0.1% SDS), The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive plus mass spectrometer.

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Zewen Wen  

PROVIDER: PXD040923 | iProX | Thu Mar 16 00:00:00 GMT 2023


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