Project description:To investigate the regulatory mechanism of Dhx37 in Sertoli cells, snRNA-seq analysis was performed on testicular cells isolated from whole testes of wild-type and Dhx37-/- mice (CKO)at P60. After quality control, single-cell transcriptomes for both wild-type and Dhx37-/- mice were obtained. UMAP dimension reduction and visualization categorized the cells into ten distinct clusters: Round Spermatids, Elongating Spermatids, Spermatocytes, Spermatogonia, Leydig cells, Fibroblasts, Sertoli cells, Macrophages, Endothelial cells, and others, based on known marker genes.
Project description:The aim of the study was to elucidate the role of Alkbh1 by targeted deletion in C57/BL6 mice. Alkb deficiency results in sex-ratio distortion of offspring and apoptosis in adult testes, most likely caused by defects in the pachytene stage during spermatogenesis. Due to the pivotal role of Alkbh1 in mouse survival and potentially in germ cells, we searched for Alkbh1-regulated genes in adult testes. Testes from 12 week old mice were selected for RNA extraction and hybridization on Affymetrix microarrays. We used testes from 3 wild type mice and 3 KO mice
Project description:Analysis of Ubb knockout mouse testes at 7, 4, 21, and 28 dpp. Ubiquitin (Ub) is an essential protein found in all eukaryotic cells and plays important roles in a variety of cellular functions including germ cell development. Targeted disruption of the polyubiquitin gene Ubb results in male and female infertility in mice with germ cells arrested at meiotic prophase I. Whole testes from wild-type (WT) and Ubb−/− (KO) mice were harvested at 7, 14, 21, and 28 days postpartum. Total RNA was extracted and hybridized to Affymetrix Mouse Genome 430 2.0 arrays.
Project description:To futher find the gene expression due to the aromatase overexpression in the mouse testis,we have employ whole genome microarray expression profiling as a discovery platform to identify genes compared to the wild type mice. The testes were taken from 5 month old aromatase overexpression mice and WT mice as control.
Project description:The ability of male reproduction is seriously dependent on Sertoli cells. However, the mechanisms governing the functional integrity of Sertoli cells remained largely unexplored. Tyrosine phosphatase protein Shp2 is expressed in germ, Leydig and Sertoli cells of mice testes. But the physiological role of Shp2 in the spermatogenesis was not fully understood. Thus, we conditionally deleted Shp2 gene in Sertoli cells using two transgenic models, and demonstrated that Shp2 deficiency caused infertility, excessive differentiation of SSCs and abnormal BTB in mice. To further discover the underlying mechanism of Shp2 regulation, we collected the mRNA of testes from wild type or knockout mice at 16.5 fetal day, Postnatal 3 days, 1 weeks, 2 weeks, and then screened the gene expression.
Project description:The global proteome profiles of wild type (wt) and Prss37-null mice in testis were compared employing data independent acquisition (DIA) technology
Project description:To futher find the gene expression due to the aromatase overexpression in the mouse testis,we have employ whole genome microarray expression profiling as a discovery platform to identify genes compared to the wild type mice. The testes were taken from 5 month old aromatase overexpression mice and WT mice as control. We investigated genes that were significantly upregulated or downregulated in AROM+ mouse testis compared with WT testis. Two groups: 1) total RNA from three 5 month old AROM+ overexpression transgenic mice testes were collected and processed for microarray;2) total RNA from three 5 month old WT mice testes as control.
Project description:To investigate the regulation of pathogenic Th2 (pTh2) cells and the role of histone deacetylase 1 (HDAC1) in the respective processes, we used a new in vitro protocol for generating pTh2 cells and performed qunatitative mass spectrometry analysis based on TMTpro 16plex multiplexing to analyze proteomes. We profiled classical Th2 and pTh2 cells from wild type (WT) mice and mice with a T cell-specific deletion of HDAC1 (HDAC1-cKO).
Project description:Sohlh1 and Sohlh2 encode a germ cell-specific basic helix-loop-helix transcriptional regulator critical in spermatogonial differentiation. Seven-day-old Sohlh1 or Sohlh2 knockout and wild-type testes were arrayed on the Affy 430 2.0 platform. The following mice were analyzed at postnatal day 7: wild-type, Sohlh1-/-, and Sphlh2-/-. 4 samples/group.