Dataset Information

Proteomic profiling reveals the impact of surface functionalization on the protein corona formed on superparamagnetic iron oxide nanoparticles

ABSTRACT: The protein corona formed on the surface of nanomaterials is critical to understanding their biological behavior, as it is regulated by both the physicochemical properties of the nanomaterials and the physiological environment. The interplay between material synthesis characteristics, protein corona composition, and biological fate remains to be fully elucidated. In this project, we investigated the protein corona of six types of 30 nm superparamagnetic iron oxide nanoparticles (IONPs), each coated with dextran and modified with different functional groups (hydroxyl, amine, and carboxyl). Proteomic analysis revealed that nanoparticles with the same surface modifications had similar protein corona compositions, while those with different surface modifications exhibited distinct complement protein profiles. These findings provide a theoretical basis for understanding the immunological responses induced by each nanoparticle type, offering insights into their biological and therapeutic applications.

ORGANISM(S): Homo Sapiens

SUBMITTER: Fangfang Chen

PROVIDER: PXD056769 | iProX | Mon Oct 14 00:00:00 BST 2024

REPOSITORIES: iProX

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Publications

Specific surface-modified iron oxide nanoparticles trigger complement-dependent innate and adaptive antileukaemia immunity.

Li Yuanyuan Y Wu Wen W Liu Qihui Q Wu Qiong Q Ren Ping P Xi Xi X Liu Haiyan H Zhao Jiarui J Zhang Wei W Wang Zizhun Z Lv Yuanyuan Y Tian Bin B Sun Shuang S Cui Jiaqi J Zhao Yangyang Y Wu Jingyuan J Gao Mingyuan M Chen Fangfang F

Nature communications 20241129 1

Considerable advances have been achieved in the application of nanomaterials for immunotherapies, yet the precise immune effects induced by protein corona remain elusive. Here, we explore the formation mechanism and immune regulation process of protein corona in acute myeloid leukaemia (AML) mouse models using commercialized iron oxide nanoparticles (IONPs), with different surface modifications, including an FDA-approved variant. Using macrophages depleted or Complement Component 3 (C3) knockout ...[more]

PMID: 39613769

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Project description:Over the last few decades, there has been a steady rise in the utilization of nanoparticles in the industry, agriculture, and health sectors. Nanoparticles have entered the food chain through air, soil, and water. However, lot of studies have been done since 2007 to explore the effect of the biological interaction of nanoparticles and its effect on drug delivery using animal models. Yet, the molecular mechanism of NP in biological systems is not clearly understood. The persistence of unstable NPs in the food web owing to decreased mobility is a major concern. However, green-synthesized nanomaterials are recognized as a relatively safer option. Therefore, in this study, both chemically and green synthesized AuNPs were synthesized, equilibrated, and compared for their colloidal stability and interaction with Brassica juncea leaf proteins. This study focuses on the comparative analysis of Chemically (Chem-AuNPs) and green synthesized gold nanoparticle (Green-AuNP) protein corona complex, specifically analyzing the differences between the soft and hard corona and their role in nanoparticle stability and homeostasis. They were incubated with Brassica juncea leaf crude for different time periods (1hr– 60hr), allowing proteins to adsorb onto the nanoparticle surface, forming a corona that influences cellular interactions, biodistribution, and physiology of plants. We characterized the protein composition in both the soft (loosely bound) and hard (tightly bound) corona using mass spectrometry, identifying key protein classes that mediate these interactions. Our findings suggest that certain protein groups, particularly those related to energy-yielding pathways bind to the corona proteins. Additionally, we observed distinct differences in protein composition between the soft and hard corona of both Chem- and Green-synthesized AuNPs, with the latter exhibiting more proteins that provide them thermodynamic stability. This work provides insights into the protein corona's role in modulating the activity of enzymes (Carbonic anhydrase, Malate dehydrogenase, and Fructose bis-phosphate aldolase) thus impacting the physiology of plants. The study also focuses on the interaction of AuNPs with RuBisCO that led to β-sheet domination in its secondary structure caused by α-helix to β- sheet transition.

Dataset Information

Proteomic profiling reveals the impact of surface functionalization on the protein corona formed on superparamagnetic iron oxide nanoparticles

Publications

Specific surface-modified iron oxide nanoparticles trigger complement-dependent innate and adaptive antileukaemia immunity.

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