Proteomics

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Proteomic profiling reveals the impact of surface functionalization on the protein corona formed on superparamagnetic iron oxide nanoparticles

ABSTRACT: The protein corona formed on the surface of nanomaterials is critical to understanding their biological behavior, as it is regulated by both the physicochemical properties of the nanomaterials and the physiological environment. The interplay between material synthesis characteristics, protein corona composition, and biological fate remains to be fully elucidated. In this project, we investigated the protein corona of six types of 30 nm superparamagnetic iron oxide nanoparticles (IONPs), each coated with dextran and modified with different functional groups (hydroxyl, amine, and carboxyl). Proteomic analysis revealed that nanoparticles with the same surface modifications had similar protein corona compositions, while those with different surface modifications exhibited distinct complement protein profiles. These findings provide a theoretical basis for understanding the immunological responses induced by each nanoparticle type, offering insights into their biological and therapeutic applications.

ORGANISM(S): Homo Sapiens

SUBMITTER: Fangfang Chen  

PROVIDER: PXD056769 | iProX | Mon Oct 14 00:00:00 BST 2024

REPOSITORIES: iProX

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Publications

Specific surface-modified iron oxide nanoparticles trigger complement-dependent innate and adaptive antileukaemia immunity.

Li Yuanyuan Y   Wu Wen W   Liu Qihui Q   Wu Qiong Q   Ren Ping P   Xi Xi X   Liu Haiyan H   Zhao Jiarui J   Zhang Wei W   Wang Zizhun Z   Lv Yuanyuan Y   Tian Bin B   Sun Shuang S   Cui Jiaqi J   Zhao Yangyang Y   Wu Jingyuan J   Gao Mingyuan M   Chen Fangfang F  

Nature communications 20241129 1

Considerable advances have been achieved in the application of nanomaterials for immunotherapies, yet the precise immune effects induced by protein corona remain elusive. Here, we explore the formation mechanism and immune regulation process of protein corona in acute myeloid leukaemia (AML) mouse models using commercialized iron oxide nanoparticles (IONPs), with different surface modifications, including an FDA-approved variant. Using macrophages depleted or Complement Component 3 (C3) knockout  ...[more]

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