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Integrated Proteomic Profiling of Aqueous Humor Reveals CDC42/RHOA-Mediated Pathogenic Mechanisms in nAMD and PDR

ABSTRACT: Neovascular AMD (nAMD) and proliferative diabetic retinopathy (PDR) are major blinding retinal diseases characterized by pathological angiogenesis. Using data-independent acquisition (DIA) proteomics, we analyzed aqueous humor from nAMD, PDR, and cataract control patients. Among 3186 identified proteins, 877 (nAMD) and 1017 (PDR) differentially expressed proteins (DEPs) were detected. Functional enrichment highlighted inflammatory and metabolic pathway dysregulation in both diseases. CDC42 and RHOA were consistently upregulated and implicated in cytoskeletal remodeling and angiogenesis. This study reveals shared molecular mechanisms in nAMD and PDR, proposing new targets for therapeutic intervention.

ORGANISM(S): Homo Sapiens

SUBMITTER: Chen Zhao

PROVIDER: PXD068217 | iProX | Tue Sep 09 00:00:00 GMT+01:00 2025

REPOSITORIES: iProX

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Integrated proteomic profiling of aqueous humor reveals CDC42/RHOA-mediated pathogenic mechanisms in nAMD and PDR.

Kong Lingjie L Qi Siyi S Han Xiaoyan X Zhang Jingyue J Zhang Linyu L Li Duo D Jiang Qin Q Zhang Shujie S Zhao Chen C

Experimental eye research 20260117

Neovascular age-related macular degeneration (nAMD) and proliferative diabetic retinopathy (PDR), though clinically distinct, are both sight-threatening ocular disorders driven by pathological neovascularization. However, their shared molecular mechanisms remain poorly characterized. In this study, we performed DIA quantitative proteomic analysis of aqueous humor (AH) from patients with nAMD, PDR, and control. A total of 3186 proteins were identified, in which 877 and 1017 differentially express ...[more]

PMID: 41554422

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