Project description:Mitogen-activated protein kinases usually affect biological processes by phosphorylating proteins. To further investigate the consequences of MAPK11 interacting with SnRK1 in controlling tomato seed dormancy, we performed a phosphorylation label-free quantitative proteomic assay in vivo with dry seeds of TS-9and MAPK11-OE lines. Data analysis showed that a total of 8261 phosphosites and 6026 phosphopeptides derived from 2711 phosphoproteins were identified. The phosphopeptides with an average fold-change >2 or <0.5 and a P value <0.05 were selected as being significantly different. Among all identified phosphopeptides, 87 phosphopeptides were significantly up-regulated and 83 phosphopeptides were significantly down-regulated in seeds of MAPK11-OE line. In addition, we also found that 348 phosphopeptides were just abundant in MAPK11-OE-13 line, and 221 phosphopeptides merely in TS-9, respectively. To explore potential biological function of MAPK11, we conducted GO function annotation and enrichment, KEGG passway annotation and enrichment, and protein protein interaction network (PPI) analyses, and detected that MAPK11 might have effect on binding, catalytic activity, transporter activity, molecular function regulator and signal transducer; and involved in cellular process, metabolic process, biological regulation, regulation of biological process and response to stimulus
Project description:Light environment provides signals for plants to develop and accomplish their life cycle successfully. Those signals are perceived and transduced by photoreceptors. Phosphorylation is one of the biochemical mechanisms initiating light signalling cascade and is a challenging question in the photobiology field today. Here, we study early light-induced phosphoproteome in Arabidopsis thaliana through a Label free LC-MS/MS proteomic approach to identify proteins which significantly change their phosphorylation status in a light-responsive way. And investigate the participation of the photoreceptors during light-dependent phosphoroylation changes.
Project description:Proteomic analysis of hippocampal tissues in control and surgery groups of mice was conducted to investigate the effects of surgery on mice at the protein expression level. The findings aim to provide insights into potential mechanisms underlying cognitive impairment following surgery.
Project description:The intent was to study, from transcriptome analysis, shade and drought responses in Solanum tuberosum (potato). We performed Illumina 50 bp single-end RNA-seq in tissues of control and treated var. Spunta wild-type plants. Drought experiments also included two independent AtBBX21-overexpressing (BBX21-OE) potato lines.
Project description:Phosphoproteome analysis of clinical tissue samples is important in the precision medicine research. Therefore, we used LC-MS/MS to systematically analyze the differential phosphorylation levels of 20 ESCC tumor tissues, paired normal tissue and lymph node metastatic tissue.
Project description:GmMYB176, an R1 MYB transcription factor regulates isoflavonoid biosynthesis in soybean. In the current experiment, GmMYB176 was silenced (GmMYB176-Si) or overexpressed (GmMYB176-OE) in soybean hairy roots and their effect on transcriptome was studied. RNA-Seq analyses of GmMYB176-Si and GmMYB176-OE along with control non-transformed soybean hairy roots revealed that alteration of gene expression of GmMYB176 affects gene regulation of hundreds of genes in soybean.