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Effect of preparation method on the protein profile of equine amnion dressings

ABSTRACT: The protein content of amnion is thought to be the primary contributor to its efficacy as a biological dressing for wounds. Protein elution into antibiotic processing media has been reported, but the effect of antiseptic-based processing methods is unknown. Here, proteomic analyses of equine amnion tissue were done to determine how two processing methods affect the protein profile when compared to unprocessed controls. Isobaric labeling combined with an SPS MS3 method implemented on an Orbitrap Fusion mass spectrometer were used to provide quantitative data.

ORGANISM(S): Cellular Organisms

SUBMITTER: Annette McCoy

PROVIDER: PXD012249 | JPOST Repository | Wed Jan 08 00:00:00 GMT 2020

REPOSITORIES: jPOST

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Effect of Preparation Method on the Protein Profile of Equine Amnion Dressings.

McCoy Annette M AM Arrington Justine J Yau Peter M PM

Journal of proteome research 20190529 6

The protein content of amnion is thought to be the primary contributor to its efficacy as a biological dressing for wounds. Protein elution into antibiotic processing media has been reported, but the effect of antiseptic-based processing methods is unknown. Amniotic membranes were collected from eight healthy mares. Samples were collected after removal of gross debris. Tissues were subsequently divided and processed with either 0.05% chlorhexidine or 2% iodine/0.25% acetic acid. After protein ex ...[more]

PMID: 31117638

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