Dataset Information

A Diecast Mineralization Process Forms the Tough Mantis Shrimp Dactyl Club

ABSTRACT: Biomineralization, the process by which mineralized tissues grow and harden via biogenic mineral deposition, is a relatively lengthy process in many mineral-producing organisms,resulting in challenges to study the growth and biomineralization of complex hard mineralized tissues. In this study we used the dactyl club of stomatopods (mantis shrimps)as a model structure to study the entire formation of hard and tough mineralized appendages. Proteomics analysis was performed prior mineralization process on freshly molted cuticle.

ORGANISM(S): Cellular Organisms

SUBMITTER: Ali Miserez

PROVIDER: PXD013153 | JPOST Repository | Wed Mar 20 00:00:00 GMT 2019

REPOSITORIES: jPOST

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Publications

A diecast mineralization process forms the tough mantis shrimp dactyl club.

Amini Shahrouz S Tadayon Maryam M Loke Jun Jie JJ Kumar Akshita A Kanagavel Deepankumar D Le Ferrand Hortense H Duchamp Martial M Raida Manfred M Sobota Radoslaw M RM Chen Liyan L Hoon Shawn S Miserez Ali A

Proceedings of the National Academy of Sciences of the United States of America 20190411 18

Biomineralization, the process by which mineralized tissues grow and harden via biogenic mineral deposition, is a relatively lengthy process in many mineral-producing organisms, resulting in challenges to study the growth and biomineralization of complex hard mineralized tissues. Arthropods are ideal model organisms to study biomineralization because they regularly molt their exoskeletons and grow new ones in a relatively fast timescale, providing opportunities to track mineralization of entire ...[more]

PMID: 30975751

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Project description:Within just a few years single cell RNA sequencing has become a frequently used standard method in many research fWithin just a few years single cell RNA sequencing has become a frequently used standard method in many research facilities worldwide. Declining sequencing costs and further refinements of the available protocols will pave the way for previously unimaginable prospects, up to single cell transcriptomic maps of entire organisms. The sample collection process can constitute a severe bottleneck in scRNA-seq experiments especially for solid tissues. Lengthy dissociation protocols are not uncommon to obtain sufficient amounts of starting material and can lead to significant changes in the gene expression profiles of cells. Preservation of the transcriptome prior to single cell dissociation can overcome this setback. Here we present an extensive performance analysis of glyoxal as an alternative fixative for scRNA-seq application. High numbers of transcripts and genes were recovered from glyoxal-fixed cells subjected to Drop-seq methodology with the best performance in Drosophila cells. While glyoxal fixation in Drosophila Kc167 and human HEK 293T cells revealed transcriptome data similar to the unfixed condition, reduced library complexity was observed for the human sample. We found the integrity of Drosophila intestinal tissue maintained following glyoxal fixation, while dissociation of the fixed tissue allowed sufficient cell isolation. In conclusion, we present glyoxal as a well-suited fixative for Drosophila samples that allows high-quality single cell transcriptomic analysis and successful intestinal tissue disaggregationcilities worldwide. Declining sequencing costs and further refinements of the available protocols will pave the way for previously unimaginable prospects, up to single cell transcriptomic maps of entire organisms. The sample collection process can constitute a severe bottleneck in scRNA-seq experiments especially for solid tissues. Lengthy dissociation protocols are not uncommon to obtain sufficient amounts of starting material and can lead to significant changes in the gene expression profiles of cells. Preservation of the transcriptome prior to single cell dissociation can overcome this setback. Here we present an extensive performance analysis of glyoxal as an alternative fixative for scRNA-seq application. High numbers of transcripts and genes were recovered from glyoxal-fixed cells subjected to Drop-seq methodology with the best performance in Drosophila cells. While glyoxal fixation in Drosophila and human cells revealed transcriptome data similar to the unfixed condition, reduced library complexity was observed for the human sample and might require further protocol optimization. We found the integrity of Drosophila intestinal tissue maintained following glyoxal fixation, while dissociation of the fixed tissue allowed sufficient cell isolation. In conclusion, we present glyoxal as a well-suited fixative for Drosophila samples that allows high-quality single cell transcriptomic analysis and successful intestinal tissue disaggregation.

Dataset Information

A Diecast Mineralization Process Forms the Tough Mantis Shrimp Dactyl Club

Publications

A diecast mineralization process forms the tough mantis shrimp dactyl club.

Similar Datasets

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