Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

In vivo phosphorylation of TOC1

ABSTRACT: Myc-tagged N-terminal portion of TOC1 or TOC1 mutant (S194A, S201A, T204A) were expressed in tobacco leaves and then immunoprecipitated. The immunoprecipitated samples were separated on SDS-PAGE gels, and then phosphorylation sites of TOC1 were identified from gel bands.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Hirofumi Nakagami

PROVIDER: PXD026261 | JPOST Repository | Mon Oct 04 00:00:00 BST 2021

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Similar Datasets

Identification of FBP1 binding proteins in wild-type, G164D and F194S comparisons by LC-MS/MS analysis

Project description:Myc-tagged FBP1-WT, Myc-tagged FBP1-G164D, Myc-tagged FBP1-F194S or empty vector were transfected into FBP1-KO HepG2 cells. FBP1 complexes were immunoprecipitated with anti-Myc beads and then separated by SDS‒PAGE. Bands were removed from the gel, and trypsin-digested samples were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) using an Orbitrap Velos Pro (Thermo Scientific). MS/MS dataset analysis was performed using the Mascot software program (Matrix Science).

2023-05-20 | PXD042364 | JPOST Repository

PrimPol-phosphoproteomics by IP-GeLC-MS/MS

Project description:Myc-tagged PrimPol expressed in HEK-293T cells was immunoprecipitated and separated by SDS-PAGE. Protein bands were excised and subjected to in-gel tryptic digestion and phosphopeptide enrichment by Fe-IMAC.

2023-08-22 | PXD043419 | JPOST Repository

TATDN2 Resolution of R-Loops is Required for Survival of BRCA1-Mutant Cancer Cells

Project description:Immunoprecipitated protein using Myc-tagged TATDN2 from U2OS.

2023-06-16 | MSV000092192 | MassIVE

CC2_Navarre_14028srpoSlowhns-_IP_pHNStagged_antiHA

Project description:array analysis of immunoprecipitated DNA from tagged HNS proteins in 14028s mutant strain (rpoS low, hns minus) Keywords: ChIP-chip

2006-05-24 | GSE4881 | GEO

In vivo and in vitro Genome wide binding (ChIP-Seq) of Bapx1 and Sox9

Project description:Bapx1 was endogenously tagged with S-Peptide and dissected vertebral columns from these tagged embryos at E12.5 were subjected to immunoprecipitation by S-peptide antibody. Simultaneously V5 tagged Bapx1 was also over expressed in NIH_3T3 cells and immunoprecipitated with V5 antibody. Concurrently vertebral column fro E12.5 mouse embryos were subjected to immunoprecipitation with Sox9 antobody to compare binding sites of Sox9 and BApx1 in the vertebral column of developing mouse embryo

2015-03-19 | GSE36672 | GEO

ChIP-Seq of constitutively expressed Ndt80-Myc in P. pastoris

Project description:The purpose of this experiment was to identify the genes bound by Ndt80 in P. pastoris when constitutively expressed. Ndt80 was tagged with c-myc, the native Ndt80 promoter was replaced with the E. gossypii Tef1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown unti log-phase in YPD. Each experiment was repeated twice and sequenced on an Illumina HiSeq 2500.

2017-05-01 | GSE90663 | GEO

ChIP-Seq of constitutively expressed Ndt80A-Myc and Ndt80B-Myc in S. stipitis

Project description:The purpose of this experiment was to identify the genes bound by the two Ndt80 paralogs in S. stipitis when constitutively expressed. Both paralogs were tagged with c-myc, the native Ndt80 promoter was replaced with the E. gossypii Tef1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown unti log-phase in YPD. Each experiment was repeated twice and sequenced on an Illumina HiSeq 2500.

2017-05-01 | GSE90664 | GEO

ChIP-Seq of Ndt80-Myc in S. cerevisiae during meiosis and sporulation

Project description:The purpose of this experiment was to identify the genes bound by Ndt80 in S. cerevisiae during meiosis and sporulation. Ndt80 was tagged with c-myc and the protein was immunoprecipitated with a c-myc antibody. Cells were grown in liquid YPA (2% Peptone, 2% Potassium Acetate, 1% Yeast Extract) at room temperature for 22 hours. Each experiment was repeated twice and sequenced on an Illumina HiSeq 4000.

2017-05-01 | GSE90661 | GEO

ChIP-Seq of constitutively expressed Ndt80-Myc in S. cerevisiae

Project description:The purpose of this experiment was to identify the genes bound by Ndt80 in S. cerevisiae when constitutively expressed, for the native Ndt80 as well as a heterologous Ndt80 from Pichia pastoris. For the ChIPs on the native Ndt80s, Ndt80 was tagged with c-myc; for the heterologous ChIP P. pastoris Ndt80 was integrated into the S. cerevisiae genome and tagged with c-myc. In both cases, the native Ndt80 promoter was also replaced with an A. gossypii Gal1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown overnight in SRaffinose until log-phase, then in SRaffinose + 1.7% Galactose for 5 hours. Each experiment was repeated twice and sequenced on an Illumina HiSeq 4000.

2017-05-01 | GSE90660 | GEO

Identifying RasGAP SH2 binding partners in Drosophila using mass spectrometry

Project description:Drosophila S2 cells were transfected with RasGAP(WT), RasGAP(SH2*32*) and GFP control. 4 mg of S2 cell lysate was used to pulldown LAP and GFP tagged constructs using GFP-Trap. The pulled down fraction was loaded on SDS-PAGE gels and coomassie stained before several bands (a-u) from two independent but parallel experiments were excised and subjected to MS analysis.

2014-05-20 | PXD000176 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data