Proteomics

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Lambert_AP_SWATH_P93_VS8_iTRAQ

ABSTRACT: This submission is part of the mass spectrometry datasets for the manuscript by Lambert et al. that describes the use of SWATH to rapidly monitor differential interactomes. This submission contains 3 IDA files that contain iTRAQ data for the following baits: CDK4 WT, CDK4 R24C and CDK4 R24H and a negative control (GFP). Three biological replicates for each bait were analyzed. See the README file within "Methods and Protocols" and the accompanying File description. Additional details and files are found on prohits-web.lunenfeld.ca.

PROVIDER: MSV000078459 | MassIVE | Thu Jul 04 00:00:00 BST 2013

REPOSITORIES: MassIVE

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Mapping differential interactomes by affinity purification coupled with data-independent mass spectrometry acquisition.

Lambert Jean-Philippe JP   Ivosev Gordana G   Couzens Amber L AL   Larsen Brett B   Taipale Mikko M   Lin Zhen-Yuan ZY   Zhong Quan Q   Lindquist Susan S   Vidal Marc M   Aebersold Ruedi R   Pawson Tony T   Bonner Ron R   Tate Stephen S   Gingras Anne-Claude AC  

Nature methods 20131027 12

Characterizing changes in protein-protein interactions associated with sequence variants (e.g., disease-associated mutations or splice forms) or following exposure to drugs, growth factors or hormones is critical to understanding how protein complexes are built, localized and regulated. Affinity purification (AP) coupled with mass spectrometry permits the analysis of protein interactions under near-physiological conditions, yet monitoring interaction changes requires the development of a robust  ...[more]

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