Project description:4-week-old male mice were infected with 50,000 CL+luc parasites or remained uninfected. Urine was collected pre-infection and 4 days after infection. 7 days post infection, mice were treated with 0.06% carnitine in drinking water ad libitum or with benznidazole 100 mg/kg ip for 10 days. 17 days post infection, urine was collected and euthanized. Metabolites were extracted from urine in methanol and untargeted LCMS analysis ran on a Q Exactive plus instrument using a Phenomenex Luna Omega Polar C18 column.
Project description:<p>18 Bacteroidetes isoaltes were analyzed for their neuroactive potentail using un-targeted and targeted metabolomics. The bacteria were grown to stationary phase, the supernatant was separated and purified using 0.22 um filter. The metabolome from the supernatant was extracted using standard Methanol extraction. The reconstituted supernatant samples were transferred into LC vials and loaded onto a sample tray in the nanoLC coupled to the Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific). Chromatographic separation of metabolites was performed on a Proxeon EASY nLC II System (Thermo Fisher Scientific) equipped with a Thermo Scientific Acclaim PepMap RSLC C18 column (P/N ES800A). The Xbridge BEH Amide (2.5 μm, 2.1 x 150 mm, Waters, Milford, MA, USA) column was used for metabolite separation.</p>
Project description:ddMS2 run of mouse lung tissue and plasma extract using C8 column in 7.5-minute gradient and positive polarity mode in Q Exactive plus.
Project description:We compared the performance of multidimensional protein identification (MudPIT) on Velos Pro Orbitrap (VPO) and Velos Orbitrap Elite (VOE) mass spectrometers to single dimension reversed phase (RP) chromatography on a Q Exactive Plus (QE+) and an Orbitrap Fusion™ Lumos™ (OFL). Using a digested HeLa cell protein extract, we carried out 16 different chromatography conditions on four different instrumentation platforms with three replicates of each condition, for a total of 48 proteomics analyses. We first compared selected chromatography conditions of the QE+ and OFL by varying column lengths, inner diameters, and C18-RP particle sizes. We next selected one chromatography condition on each system and varied the effective RP gradients lengths
Project description:Plasma and Cerebrospinal fluid data were acquired on the Q-Exactive with 3 different columns: a C8 column, a C18 column, and a polar C18 Column.
Project description:MS/MS fragmentation data of the compounds acquired on Q Exactive - with
chromatographic separation on a Phenomenex polar C18 column.
Project description:MS/MS fragmentation data of synthetic standards acquired on Q Exactive - with chromatographic separation on a Phenomenex polar C18 column.