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Monkeypox and vaccinia virus infection of primary human astrocytes

ABSTRACT: Primary human astrocytes were infected with either monkeypox virus (MPXV clade IIb lineage), vaccinia virus (VACV: Acambis 2000), or controls (MC=monkeypox control, AC = Vaccinia control) at an MOI of 10 for 6 h. Samples (n=4) were analyzed by LC-MS/MS with label-free quantification where the data was acquired by data-dependent acquisition (DDA).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Olivier Julien

PROVIDER: MSV000092229 | MassIVE | Wed Jun 21 11:58:00 BST 2023

REPOSITORIES: MassIVE

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Monkeypox virus infection of human astrocytes causes gasdermin B cleavage and pyroptosis.

Miranzadeh Mahabadi Hajar H Lin Y C James YCJ Ogando Natacha S NS Moussa Eman W EW Mohammadzadeh Nazanin N Julien Olivier O Alto Neal M NM Noyce Ryan S RS Evans David H DH Power Christopher C

Proceedings of the National Academy of Sciences of the United States of America 20240212 8

Monkeypox virus (MPXV) infections in humans cause neurological disorders while studies of MPXV-infected animals indicate that the virus penetrates the brain. Pyroptosis is an inflammatory type of regulated cell death, resulting from plasma membrane rupture (PMR) due to oligomerization of cleaved gasdermins to cause membrane pore formation. Herein, we investigated the human neural cell tropism of MPXV compared to another orthopoxvirus, vaccinia virus (VACV), as well as its effects on immune respo ...[more]

PMID: 38346199

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