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PEPPI-SP3: Robust and Simple Sample Prefractionation Workflow for Top-Down Proteomics

ABSTRACT: Precise prefractionation of proteome samples is a potent method for realizing in depth analysis in top-down proteomics. PEPPI MS (Passively Eluting Proteins from Polyacrylamide gels as Intact species for MS), a gel-based sample fractionation method, enables high-resolution proteome fractionation based on molecular weight by highly efficient extraction of proteins from polyacrylamide gels after SDS PAGE separation. In this study, we developed a complete, robust, and simple sample preparation workflow named PEPPI SP3 for top down proteomics by combining PEPPI MS with the magnetic bead-based protein purification approach used in SP3.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Luca Fornelli

PROVIDER: MSV000095992 | MassIVE |

REPOSITORIES: MassIVE

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