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To Study effect of Bisphenol A mitochondrial proteins through LCMS

ABSTRACT: HepG2 cells were maintained in DMEM media with 10 percent FBS under standard conditions of 37 degree Celsius, 5 percent CO2. Cells were then treated with Bisphenol A at various concentrations (1, 10, 100 micromolar) for 48 hrs. Mitochondrial proteins were isolated following differential centrifugation method (bioprotocol.org/e1809). Quantification of the mitochondrial proteins was done using the BCA reagent. Isolated proteins (100microgram), trypsin digested were then proceeded for LCMS analysis as detailed earlier (PMID 39216816). Label free quantification is done without any PTMs. The study protocol is approved by the competent authority of the institute (vide number: IN_IEC_SPL_75). The team acknowledges PGIMER Chandigarh for providing financial assistance (IM213_0_28-08-23_1123, Structure activity relationship of Bisphenol A on lysosomal Enzymes- A step towards understanding plastic induced Nonalcoholic fatty liver disease).

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Human

SUBMITTER: Prof DIBYAJYOTI BANERJEE

PROVIDER: MSV000097672 | MassIVE | Mon Apr 21 02:21:00 BST 2025

REPOSITORIES: MassIVE

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