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Engineered Probiotics Overexpressing Lactate Dehydrogenase Suppress Colorectal Cancer Tumorigenesis

ABSTRACT: This dataset contains GC-MS metabolomics profiles of bacterial culture supernatants from both engineered and wild-type probiotic strains. Specifically, the strains include Bifidobacterium longum pBS423, B. longum pBS423-ldh, Lactobacillus plantarum pLH01, pLH01-ldh, and wild-type strains of B. longum subsp. infantis, B. longum subsp. longum, B. bifidum, L. plantarum, and Escherichia coli. Culture supernatants were collected, filtered (0.22 um), vacuum-dried, and derivatized using methoxyamine hydrochloride and BSTFA. GC-MS analysis was performed using a Thermo Trace 1310 gas chromatograph coupled to a Thermo ISQ LT single quadrupole mass spectrometer. The dataset includes raw data files in NetCDF (.cdf) format, processed peak tables, metabolite identification results, and experimental metadata. Quantitative analysis was performed across biological replicates, and standard compounds such as indole-3-lactic acid and 3-phenyllactic acid were included at multiple concentrations for calibration. Quality control (QC) samples were analyzed periodically to ensure analytical reproducibility.

INSTRUMENT(S): Thermo Trace 1310 GC-MS with ISQ LT (single quadrupole)

ORGANISM(S): Lactobacillus Plantarum (ncbitaxon:1590) Bifidobacterium Longum Subsp. Infantis (ncbitaxon:1682) Escherichia Coli (ncbitaxon:562) Bifidobacterium Longum Subsp. Longum Jcm 1217 (ncbitaxon:565042) Bifidobacterium Bifidum Dsm 20456 = Jcm 1255 (ncbitaxon:500634)

SUBMITTER: Minhye Shin

PROVIDER: MSV000097780 | MassIVE | Fri May 02 00:32:00 BST 2025

REPOSITORIES: MassIVE

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