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Li et al., Xylosyltransferase Bump-and-hole Engineering to Chemically Manipulate Proteoglycans in Mammalian Cells: Decorin glycosylated in-cell with 6AzGlc and clicked with ITag

ABSTRACT: pgsA-745 cells stably transfected with a plasmid encoding for BH-XT1 were transfected transiently with a plasmid encoding human FLAG-tagged decorin. Cells were fed with 6AzGlc-1-phosphate precursor 1, and supernatant isolated. Decorin was purified, then clicked with ITag-alkyne, digested with trypsin on S-Trap, then analysed by HCD on an Orbitrap Eclipse. Data analysis with Byonic using 6AzGlc-ITag as a custom mod.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Benjamin Schumann

PROVIDER: MSV000098981 | MassIVE | Sat Aug 30 01:40:00 BST 2025

SECONDARY ACCESSION(S): PXD067896

REPOSITORIES: MassIVE

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