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Human Primary Airway Epithelium Response to HCoV-229E Infection

ABSTRACT: The purpose of this experiment was to evaluate the human host cellular response to wild-type Human coronavirus strain 229E (HCoV-229E) infection of primary human airway epithelial cell cultures. Sample data was obtained from mock-infected primary human airway epithelial cell cultures at 24 hours post infection. Primary human conducting airway epithelial cells were grown at air-liquid interface. Samples were harvested and processed for RNA sequencing (RNA-Seq) expression analysis and limited proteolysis proteomics. Wild-type human coronavirus strain 229E (HCoV-229E) infected and mock-infected primary human airway epithelial cell cultures with and without primary human lung macrophages were harvested at 24 hour post infection (MOI 3). Samples were processed for RNA sequencing (RNA-Seq; in Trizol) expression analysis and limited proteolysis proteomics (LiP). For LiP, cells were lysed by freeze thaw cycles and each replicate was split in half and treated for 1 minute with proteinase K and then trypsin and the other half just treated with trypsin prior to processing for proteomic analysis. Search for the DDA data was performed using MaxQuant, following the label-free quantification and match between runs (LFQ-MBR) workflow. The DIA data was searched with FragPipe/DIA-NN. The database search results were further processed using in-house codes which filtered and normalized the data, and added statistical parameters.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Human Coronavirus 229e (ncbitaxon:11137)

SUBMITTER: John T. Melchior Amy Sims

PROVIDER: MSV000099878 | MassIVE | Wed Nov 12 18:08:00 GMT 2025

REPOSITORIES: MassIVE

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