Exploring the Role of Gut Microbiota and Metabolomic Changes on Singing in Zebra Finches
Ontology highlight
ABSTRACT: Recent studies have demonstrated that the cognitive abilities of songbirds are linked to gut microbiome beta diversity. To explore whether the gut microbiome influences vocalization, a cognitive trait, we examined how antibiotics impact metabolism, microbiome and singing behavior in adult male zebra finches. Using untargeted liquid chromatography-mass spectrometry and metagenomic sequencing, we observed significant changes in stool metabolites, microbiome composition and vocal behavior after 14 days of antibiotic treatment. Metabolomic analysis revealed disruptions in the alanine, aspartate and glutamate pathways post-treatment. Furthermore, an analysis of phyla in the gut microbiome demonstrated a decrease in Firmicutes and an increase in Bacteroidetes. Treated birds sang fewer female-directed (FD) songs and produced more introductory notes compared to controls, suggesting possible alterations in neural pathways tied to motivation and vocalization. Despite the decrease in their number, the spectral and temporal properties of FD songs remained stable. Our findings suggest that antibiotic-induced changes in the gut microbiome may influence complex social behaviors such as vocal communication in zebra finches.
INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - hilic
PROVIDER: MTBLS12553 | MetaboLights | 2025-06-25
REPOSITORIES: MetaboLights
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| QC1-P.mzML | Mzml | |||
| QC1-P.raw | Raw | |||
| QC1_NEG-N.mzML | Mzml | |||
| QC1_NEG.raw | Raw | |||
| QC2-P.mzML | Mzml |
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