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Harnessing Exogenous Stimuli and Functionally 3D Aligned Fibrous Scaffolds for Spatiotemporal Modulation of Bone Regeneration

ABSTRACT:

Bone regeneration requires spatiotemporal coordination of immune modulation, stem cell recruitment, angiogenesis, and osteogenesis, yet most scaffolds lack sequential control across healing phases. We develop a near-infrared (NIR)-responsive therapeutic platform that integrates clinically available irradiation with an engineered 3D radially aligned nanofiber scaffold functionalized with black phosphorus (BP) and a bone marrow mesenchymal stem cell (BMSC)-targeting aptamer (Apt19S). NIR photothermal stimulation accelerates BP degradation, releasing phosphate ions and activating a heat-shock program to promote macrophage polarization, endogenous MSC homing, neovascularization, and osteogenic differentiation. Metabolomics reveals cooperative regulation of HSP-linked signaling and lipid metabolism. In a rat critical-size calvarial defect, the platform achieves robust bone regeneration without exogenous cells or growth factors. The system is simple, structurally tunable, and shape-customizable, providing a clinically translatable and modular framework for spatiotemporal microenvironment programming in bone and other regenerative settings.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS13974 | MetaboLights | 2026-03-04

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS13974_LC-MS_negative_reverse-phase_metabolite_profiling.txt	Txt
	a_MTBLS13974_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS13974_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular
	m_MTBLS13974_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular

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