Integrated Analysis of Gut Microbiota, Serum Metabolomics, and Proteomics Reveals Novel Associations With Clinical Symptoms in Patients With Cerebral Infarction

ABSTRACT: Cerebral infarction (CI) is a major cause of adult disability and mortality worldwide. Mounting evidence supports the critical role of the gut–brain axis in cerebrovascular disease progression. This study aimed to characterize the alterations in gut microbiota, serum metabolome, and serum proteome in patients with CI, and to identify multi-omics signatures associated with clinical symptoms. A total of 20 CI patients and 20 healthy controls (HC) were enrolled. Fecal microbiota was profiled using 16S rRNA gene high-throughput sequencing. Serum metabolomics and proteomics were analyzed using ultra-high-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) and data-independent acquisition (DIA) proteomics, respectively. Spearman correlation and multi-omics integration were applied to explore the associations among microbiota, metabolites, proteins, and clinical indicators.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS14356 | MetaboLights | 2026-05-08

REPOSITORIES: MetaboLights