Dataset Information
Multiomics analysis of neutrophils in SLE: insights from adult and pediatric disease
Ontology highlight
ABSTRACT: Neutrophils contribute to systemic lupus erythematosus (SLE) pathogenesis through ROS and NET production, and increased apoptotic debris which causes autoantibody production and immune complex formation. These processes drive inflammation and tissue damage. The aim of this study was to perform integrated transcriptomic and metabolomic analyses comparing paediatric and adult SLE neutrophils. Adult (aSLE) and pediatric (jSLE) patient and healthy control neutrophils were subjected to RNAseq and 1H-NMR metabolomics. Univariate, multivariate and multiomics enrichment analyses were conducted in R and with Ingenuity Pathway Analysis (IPA). Transcriptomic analysis revealed distinct gene expression profiles. aSLE was enriched in LTF and ARHGEF12, while jSLE showed elevated SIGLEC1, OTOF, and IRF7. MMP8, OLFM4, and IFI27 were upregulated across both SLE groups (adj.p-value<0.05). Gene Ontology analysis revealed enrichment in cell cycle and interferon signalling in aSLE, and angiogenesis and tissue-specific development in jSLE. Adult and juvenile SLE neutrophils were enriched for IFN-α/β signalling, neutrophil degranulation and NET signalling pathways (IPA, adj.p-value<0.01). Metabolomic profiling identified distinct metabolic alterations in aSLE, with a greater complexity of metabolic changes in jSLE. Multivariate PLS-DA improved group discrimination, particularly in aSLE (balanced accuracy 80%, sensitivity 80%). VIP>1 metabolites were enriched in taurine/hypotaurine and amino acid metabolism. Integrating transcriptomic and metabolomic data strengthened IFN-α/β signalling, neutrophil degranulation and NET signalling (adj. p <0.001). Additional metabolic pathways uniquely enriched in aSLE included glutamate and glutamine metabolism and nucleotide biosynthesis (adj.p<0.01). In summary, neutrophils from SLE patients, especially in jSLE, displayed complex transcriptomic and metabolic profiles, with aberrant IFN responses and neutrophil activation.
INSTRUMENT(S): Nuclear Magnetic Resonance (NMR) -
PROVIDER: MTBLS6259 | MetaboLights | 2026-04-22
REPOSITORIES: MetaboLights
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| a_MTBLS6259_NMR___metabolite_profiling.txt | Txt | |||
| i_Investigation.txt | Txt | |||
| m_MTBLS6259_NMR___metabolite_profiling_v2_maf.tsv | Tabular | |||
| s_MTBLS6259.txt | Txt |
Items per page: 1 - 4 of 4 |
Publications
Multiomics analysis of neutrophils in SLE: insights from adult and paediatric disease.
Clinical and experimental immunology 20250101 1
Neutrophils contribute to systemic lupus erythematosus (SLE) pathogenesis through reactive oxygen species and neutrophil extracellular trap (NET) production, and increased apoptotic debris which causes autoantibody production and immune complex formation. These processes drive inflammation and tissue damage. The aim of this study was to perform integrated transcriptomic and metabolomic analyses comparing paediatric and adult SLE neutrophils. Adult (aSLE) and paediatric (jSLE) patient and healthy ...[more]
