Project description:Severe thrombocytopenia can be a determinant factor in the morbidity of Plasmodium vivax (Pv), the most widespread human malaria. Although immune mechanisms may drive Pv-induced severe thrombocytopenia (PvST), the current data on the cytokine landscape in PvST is scarce, and often conflicting. The analysis of the bidirectional circuit of inflammatory mediators and miRNAs would lead to a better understanding of the mechanisms underlying PvST.
Project description:Blood samples were purified in plasma from healthy volunteers' blood and COVID-19 patients for miRNA analysis. qPCR gene expression profiling. Plasma from eight controls and patients were used. Equal amount total RNA from each controls and patients was pooled prior to gene expression analysis.