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Mechanism of Trichloroethylene (TCE) toxicity in the placenta

ABSTRACT: Cells in culture will be exposed to S-(1,2-dichlorovinyl)-L-cysteine (DCVC), a relevant metabolite of TCE. The first cell line used will be HTR-8/SVneo, originally derived from first trimester female human cytotrophoblast cells and immortalized with simian virus 40 large T antigen, this cell line models first-trimester placental extravillous trophoblasts in vitro. HTR-8/SVneo cells will be cultured for 24 hours followed by exposure to cell culture media (control) or 20 µM DCVC for 6 or 12 hours. Cells (cells frozen in cell culture dish) will then be collected (n=5) In the TCE aim of this study, we will utilize one cell model that phenotypically represents an important placental cell population.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): Cultured Cells

DISEASE(S): Environmental Exposure

SUBMITTER: Maureen Kachman

PROVIDER: ST001398 | MetabolomicsWorkbench | Mon Jun 08 00:00:00 BST 2020

REPOSITORIES: MetabolomicsWorkbench

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