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Abscisic acid (ABA) application to Populus × canescens in hydroponics

ABSTRACT: Abscisic acid (ABA) application to Populus × canescens in hydroponics

PROVIDER: PRJEB30670 | ENA |

REPOSITORIES: ENA

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Abscisic acid (ABA) application to Populus × canescens in hydroponics

Project description:4 weeks old rooted plantlets of P. × canescens (Clone INRA717 1-B4) were cultivated in hydroponics in 2 l pots in Long Ashton nutrient solution in a culture room for 8 weeks before treatments started. Three treatments were applied to the plants: control treatment (-ABA), continuous 100 µM ABA treatment (+ABA) and discontinuous 100 µM ABA treatment (±ABA). ABA was fed to +ABA plants during the whole treatment period of 30 days. ABA was fed to ±ABA plants for three days in two weeks. Developing xylem and mature xylem were collected separately during the harvest and shortly frozen in liquid nitrogen. RNA was extracted from these samples and followed by RNA-sequencing.

2019-01-20 | E-MTAB-7587 | biostudies-arrayexpress

RNA-seq analysis of Arabidopsis treated with abscisic acid (ABA) and its derivatives

Project description:Transcriptome of chemical-treated plants were investigated for the evaluation of ABA derivatives. Abscisic acid and its derivatives up-regulated mainly abiotic stress responsive genes.

2020-03-01 | GSE127910 | GEO

Transcriptome analysis of Arabidopsis thaliana G protein subunit mutants in response to abscisic acid (ABA)

2010-12-06 | GSE19520 | GEO

Transcriptional profiling by array of Arabidopsis ABA auxotroph to identify early downstream targets of transcription in response to a small dose of abscisic acid (ABA)

Project description:Abscisic acid (ABA) is a plant hormone that is important in responding to various environmental stresses. Using an ABA auxotroph in Arabidopsis (aba2-2) as the plant material, we would like to identify early downstream targets of transcription in response to a small dose of ABA, 1 uM. We also added cycloheximide to preferentially obtain immediate targets of ABA addition. We believe that using a sensitized background of an ABA auxotroph would yield a set of genes that are very closely regulated by ABA at the transcriptional level. This data set will be used for network analysis of ABA signaling.

2011-10-30 | E-MEXP-3404 | biostudies-arrayexpress

Grape skin transcriptome in the berries cultured in vitro treated with exogenous abscisic acid

2011-08-26 | GSE31662 | GEO

Grape skin transcriptome in the berries cultured in vitro treated with exogenous abscisic acid

Project description:The softened green berries of Cabernet Sauvignon were cultured in a Petri dish. 0.3M sucrose solution with (±)-abscisic acid (ABA) and without (±)-ABA (control) were applied to the Petri dishes. The berry skins were sampled at 3 and 10 days after the application. PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Kazuya Koyama. The equivalent experiment is VV17 at PLEXdb. ABA treated - time: 3 days(2-replications); ABA treated - time: 10 days(2-replications); Non-treated (control) - time: 3 days(2-replications); Non-treated (control) - time: 10 days(2-replications)

2011-08-25 | E-GEOD-31662 | biostudies-arrayexpress

Transcriptome analysis of Arabidopsis thaliana G protein subunit mutants in response to abscisic acid (ABA)

Project description:Heterotrimeric G proteins mediate crucial and diverse signaling pathways in eukaryotes. To gain insights into the regulatory modes of the G protein and the co-regulatory modes of the G protein and the stress hormone abscisic acid (ABA), we generated and analyzed gene expression in G protein subunit single and double mutants of the model plant Arabidopsis thaliana. Through a Boolean modeling approach, our analysis reveals novel modes of heterotrimeric G protein action. Keywords: transcriptome analysis; G protein subunit mutants; abscisic acid (ABA) Microarray data were generated from four genotypes (wild type, gpa1-4 mutant, agb1-2 mutant, agb1-2 gpa1-4 double mutant) with or without ABA treatment. Arabidopsis plants were grown in growth chambers with an 8 hr light/16hr dark. Three hundred Arabidopsis leaves excised from 60-70 five-week-old plants were used as the starting material for each guard cell microarray. Ten mature leaves taken from 3-4 plants grown side-by side with the plants for guard cell isolation were used for each leaf sample. Excised leaf and isolated guard cell samples were treated with ABA (50 μM) or EtOH (solvent control) for 3 hrs. For each type of sample (guard cells or leaves), three independent biological replicates were performed, resulting in a total of 48 microarray hybridizations (2 sample types ´ 4 genotypes ´ two treatments ´3 replicates).

2010-12-06 | E-GEOD-19520 | biostudies-arrayexpress

Transcription profiling of Arabidopsis srk2cf mutant plants under abscisic acid (ABA) treatment for 0, 1 and 4 hours

Project description:Microarray experiments were performed using Arabidopsis wild type plants (Col-0) and srk2cf double knockout mutants to investigate functions of two osmotic stress-activated protein kinases, SRK2C and SRK2F. Transcription profiles of wild type and mutants were compared under abscisic acid (ABA) treatment for 0, 1 and 4 h.

2009-10-15 | E-MEXP-2378 | biostudies-arrayexpress

Abscisic acid effect on wild type and the abh1 mutant

Project description:Analysis of the abh1 mutant Arabidopsis plants following treatment with 50 uM abscisic acid (ABA). ABH1 encodes the large (80kDa) subunit of the nuclear mRNA cap binding complex and affects early ABA signal transduction events (Hugouvieux et al., 2001, Cell 106, 477). Keywords: stress response

2007-03-01 | GSE7112 | GEO

Unravelling the molecular mechanisms of Abscisic acid-mediated drought-stress alleviation in pomegranate (Punica granatum L.)

Project description:Pomegranate (Punica granatum L.) is sensitive to drought stress, which largely affects its transplantation survival rate, fruit yield and quality. Abscisic acid (ABA) treatment can reduce the drought-induced adverse impacts on plants. However, no studies have ever applied ABA as an exogenous supply to alleviate the drought stress on pomegranates. In this study, we performed comparative transcriptome analysis between the ABA-treated and untreated pomegranates to reveal the ABA-induced mechanisms in response to drought-stress. Our results showed that exogenous ABA application substantially enhanced pomegranate drought resistance by strengthening metabolic pathways, such as BRs synthesis, peroxisome biogenesis, photosynthesis and hemicelluloses synthesis. Furthermore, treatments with different ABA concentrations may provoke different transcriptional responses and, once the concentration exceeds the optimal (60 μM), it might induce some potential adverse impacts on plant growth and stress resistance.

2020-10-20 | GSE156104 | GEO

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