Project description:This study addresses the response of Streptococcus pneumoniae to zinc. The wild-type strain D39 was grown in GM17 and GM17 with 0.25 mM of ZnSO4. The transcriptome under these conditions was compared by using microarrays. Keywords: transcriptome analysis of D39 wild-type compared with its isogenic glnPA double mutant
Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in CDM Plus 0mM Zn2+ to grown in CDM plus 0.2 mM Zn2+.
Project description:Transcriptome comparison of the Streptococcus pneumoniae D39∆tpxD grown in THY medium till OD620=0.25 and then treated with or without 1mM H2O2 for 40mins.
Project description:This study addresses the response of Streptococcus pneumoniae to zinc. The wild-type strain D39 was grown in GM17 and GM17 with 0.25 mM of ZnSO4. The transcriptome under these conditions was compared by using microarrays. Keywords: transcriptome analysis of D39 wild-type compared with its isogenic glnPA double mutant The wild-type strain D39 was grown in 4 biological replicates in GM17 and in GM17+0.25 mM ZnSO4 to an Optical Density at 600 nm of 0.3. From these cultures samples were prepared for transciptome determination with in-house printed microarrays covering the genomes of several S. pneumoniae species. The GM17 samples (CH1) and GM17+ZnSO4 samples (CH2) were labeled with Cy5 or Cy3. Samples were hybridized to glass-microarray slides. This work is part of a publication that is likely to be released in june/july 2008: 'Opposite effects of Mn2+ and Zn2+ on the PsaR-mediated expression of the virulence genes pcpA, prtA and psaBCA of Streptococcus pneumoniae" by Kloosterman et al.
Project description:Comparison of the Streptococcus pneumoniae D39 spxB- mutant vs D39. For DNA microarray analysis, D39 wild-type and two independently obtained D39spxB- mutants were grown as four, two and two biological replicates, respectively, in Glc-M17 under semi-aerobic conditions and harvested at an OD595 of approximately 0.25 (mid-exponential). The RNA of both D39spxB- strains was compared to D39 and the combined data was analyzed. All other procedures regarding microarray analyses were done as described before. A gene was considered differentially expressed when the fold change was ≥ 2, ≤ 0.5, with a Bayes p ≤ 0.00001 and when at least 7 measurements were available.
Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in THY medium till OD620=0.25 and then treated with or without 1mM H2O2 for 40mins.
