Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA115593 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The study of the gene expression during otic development is a source of important information for understanding the mechanism of inner ear development and subsequently create appropriate gene therapies both to prevent hearing loss and eventually to regenerate the damaged parts.This dataset contains data from temporal expression profiles in an epithelial cell line derived from the cochlear duct of a mouse inner ear at embryonic day E10.5. There are 6 time points across 14 days of in vitro differentiation in serum-free media. We used microarrays to define patterns of expression in a differentiating otic cell line. Experiment Overall Design: The model is based on the conditionally immortal cell line University of Sheffield/ventral otocyst-epithelial cell line clone 36 (US/VOT-E36), derived from ventral otic epithelial cells of the mouse at embryonic day 10.5. This cell ilines is recapitulates a coherent pattern of cell differentiation compared with in vivo cells and provide a convenient model for screening the effects of other extrinsic factors on the differentiation of cochlear epithelial cell types in vitro. The cell line VOT-E36 was weaned from the fetal calf serum (FCS), which was used to culture the parental cell line, by progressive serum dilution. The cell line was conditionally immortalized by a temperaturesensitive variant of the T antigen (tsA58), controlled by a c-interferoninducible promoter. Cell growth could then be arrested and the cells allowed to differentiate by culturing them at 39 C without c-interferon. Cultures were customarily set up under proliferative conditions (33 C, c-interferon) and allowed to grow for 2–3 days. To induce differentiation, the dishes were rinsed twice with Ultraculture and the medium was replaced without c-interferon and then transferred to 39 C. Medium was renewed every 4 days.
2009-04-17 | E-GEOD-15585 | biostudies-arrayexpress
Project description:The study of the gene expression during otic development is a source of important information for understanding the mechanism of inner ear development and subsequently create appropriate gene therapies both to prevent hearing loss and eventually to regenerate the damaged parts.This dataset contains data from temporal expression profiles in an epithelial cell line derived from the cochlear duct of a mouse inner ear at embryonic day E10.5. There are 6 time points across 14 days of in vitro differentiation in serum-free media. We used microarrays to define patterns of expression in a differentiating otic cell line. Keywords: Temporal gene expression of 14 days of differentiation in vitro.
2009-04-08 | GSE15585 | GEO
Project description:PURPOSE: To provide a detailed gene expression profile of the normal postnatal mouse cornea. METHODS: Serial analysis of gene expression (SAGE) was performed on postnatal day (PN)9 and adult mouse (6 week) total corneas. The expression of selected genes was analyzed by in situ hybridization. RESULTS: A total of 64,272 PN9 and 62,206 adult tags were sequenced. Mouse corneal transcriptomes are composed of at least 19,544 and 18,509 unique mRNAs, respectively. One third of the unique tags were expressed at both stages, whereas a third was identified exclusively in PN9 or adult corneas. Three hundred thirty-four PN9 and 339 adult tags were enriched more than fivefold over other published nonocular libraries. Abundant transcripts were associated with metabolic functions, redox activities, and barrier integrity. Three members of the Ly-6/uPAR family whose functions are unknown in the cornea constitute more than 1% of the total mRNA. Aquaporin 5, epithelial membrane protein and glutathione-S-transferase (GST) omega-1, and GST alpha-4 mRNAs were preferentially expressed in distinct corneal epithelial layers, providing new markers for stratification. More than 200 tags were differentially expressed, of which 25 mediate transcription. CONCLUSIONS: In addition to providing a detailed profile of expressed genes in the PN9 and mature mouse cornea, the present SAGE data demonstrate dynamic changes in gene expression after eye opening and provide new probes for exploring corneal epithelial cell stratification, development, and function and for exploring the intricate relationship between programmed and environmentally induced gene expression in the cornea. Keywords: other
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