Project description:This experiment highlights the extreme sequence bias generated by standard PCR amplication of sequencing libraries and decribes an adapted T7-polymerase based amplification method, which results in non-baised, representative libraries for Illumina sequencing
Project description:This experiment highlights the extreme sequence bias generated by standard PCR amplication of sequencing libraries and decribes an adapted T7-polymerase based amplification method, which results in non-baised, representative libraries for Illumina sequencing Adaptation of standard Illumina sequencing protocol to obtain representative sequencing libraries after sample amplification. Comparing different amplification methods with amplification free sequencing.
Project description:In crosslinked MINUTE-ChIP, formaldehyde-fixed chromatin is fragmented using sonication, blunted and ligated to double-stranded DNA adaptors that include a T7 RNA polymerase promoter and a sample barcode sequence. Finally, samples are combined and subsequent ChIP reactions are performed with the pooled samples. ChIP material is prepared into an Illumina-compatible library using linear amplification by virtue of T7 RNA polymerase, reverse transcription and a low-cycle library PCR amplification. Here, we demonstrate a MINUTE-ChIP for Nanog from formaldehyde-fixed cells using fragmentation by sonication.
Project description:Single-target high-throughput transcription analysis reveal high levels of alternative splicing present in the FPPS/GGPPS from Plasmodium falciparum
Project description:RNA-Seq Analysis of Splicing in Plasmodium falciparum Uncovers New Splice Junctions, Alternative Splicing, and Splicing of Antisense Transcripts
Project description:Three-dimensional modeling of the P. falciparum genome during the erythrocytic cycle reveals a strong connection between genome architecture and gene expression