[Candida] glabrata
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ABSTRACT:
PROVIDER: PRJNA169411 | ENA |
REPOSITORIES: ENA
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Project description:To delineate the interaction of Candida glabrata with host immune cells, we performed genome-wide transcriptional profiling analysis on THP-1 macrophage-internalized wild-type and chromatin remodeling defective mutant (Cgrsc3-a∆ and Cgrtt109∆) yeasts. Genes implicated in ergosterol biosynthesis, and high-affinity iron uptake and homeostasis were found to be down-regulated in C. glabrata wild-type and mutant cells upon macrophage internalization. Additionally, global gene expression profiles of RPMI-grown and macrophage-ingested Cgrsc3-a∆ and Cgrtt109∆ cells revealed down-regulation of genes involved in mitochondrial respiration under normal growth conditions and induction of genes required for generation of precursors of metabolites and energy upon macrophage internalization. To examine the behavior of Candida glabrata wild-type and chromatin remodeling defective mutants upon internalization by differentiated human monocytic THP-1 cells, we compared the transcript profiles of 10 hour RPMI-grown with those of 10 hour THP-1 macrophage internalized C. glabrata cells. Additionally, early transcriptional response of C. glabrata wild-type cells to macrophage internal milieu was examined post 2 hour THP-1 macrophage infection.
2012-08-18 | GSE38953 | GEO
Project description:To delineate the interaction of Candida glabrata with host immune cells, we performed genome-wide transcriptional profiling analysis on THP-1 macrophage-internalized wild-type and chromatin remodeling defective mutant (Cgrsc3-aM-bM-^HM-^F and Cgrtt109M-bM-^HM-^F) yeasts. Genes implicated in ergosterol biosynthesis, and high-affinity iron uptake and homeostasis were found to be down-regulated in C. glabrata wild-type and mutant cells upon macrophage internalization. Additionally, global gene expression profiles of RPMI-grown and macrophage-ingested Cgrsc3-aM-bM-^HM-^F and Cgrtt109M-bM-^HM-^F cells revealed down-regulation of genes involved in mitochondrial respiration under normal growth conditions and induction of genes required for generation of precursors of metabolites and energy upon macrophage internalization. To examine the behavior of Candida glabrata wild-type and chromatin remodeling defective mutants upon internalization by differentiated human monocytic THP-1 cells, we compared the transcript profiles of 10 hour RPMI-grown with those of 10 hour THP-1 macrophage internalized C. glabrata cells. Additionally, early transcriptional response of C. glabrata wild-type cells to macrophage internal milieu was examined post 2 hour THP-1 macrophage infection. Agilent one-color experiment, Organism: Yeast (Candida glabrata)
2012-08-18 | E-GEOD-38953 | biostudies-arrayexpress
Project description:The goal of the current study was to identify differentially-expressed genes upon CgSNF2 deletion, as well as, upon macrophage internalization in C. glabrata wild-type (wt) and Cgsnf2Δ strains. For this study, RNA samples were collected from RPMI-grown and macrophage-internalized cells of C. glabrata wild-type and Cgsnf2Δ strains at 2 h and 10 h post-infection. Comparative transcriptome analysis shows the differential regulation of 1419 genes in C. glabrata wild-type cells upon macrophage internalization, whereas the CgSNF2 deletion leads to altered regulation of 935 genes in C. glabrata wild-type cells.
2024-04-16 | GSE234670 | GEO
Project description:To examine the role of a glycosylphosphatidylinositol-linked aspartyl protease, CgYps1, in the regulation of pH homeostasis in Candida glabrata, transcriptional profiling analysis was carried out on wild-type and Cgyps1∆ cells grown in YNB medium (pH 5.5) and in YNB medium adjusted to pH 2.0. Genes involved in carbohydrate and amino acid metabolism, protein folding and stress response pathways were found to be differentially regulated in response to acidic environment in both the strains. To examine the role of a glycosylphosphatidylinositol-linked aspartyl protease, CgYps1, in the regulation of pH homeostasis in Candida glabrata, transcriptional profiling analysis was carried out on wild-type and Cgyps1∆ delta cells grown in YNB medium (pH 5.5) and in YNB medium adjusted to pH2.0. Genes involved in carbohydrate and amino acid metabolism, protein folding and stress response pathways were found to be differentially regulated in response to acidic environment in both the strains
2011-01-10 | GSE24267 | GEO
Project description:To examine the response of Candida glabrata cells to iron-depleted and iron-repleted environmental conditions, transcriptional profiling analysis was carried out on wild-type and Cghog1∆ cells grown either in presence of BPS or ferric chloride. Genes involved in iron transport and homeostasis, oxidative phosphorylation, amino acid metabolic process and chromatin silencing were found to be differentially regulated.
2015-03-21 | GSE60741 | GEO
Project description:To examine the role of a glycosylphosphatidylinositol-linked aspartyl protease, CgYps1, in the regulation of pH homeostasis in Candida glabrata, transcriptional profiling analysis was carried out on wild-type and Cgyps1∆ cells grown in YNB medium (pH 5.5) and in YNB medium adjusted to pH 2.0. Genes involved in carbohydrate and amino acid metabolism, protein folding and stress response pathways were found to be differentially regulated in response to acidic environment in both the strains. To examine the role of a glycosylphosphatidylinositol-linked aspartyl protease, CgYps1, in the regulation of pH homeostasis in Candida glabrata, transcriptional profiling analysis was carried out on wild-type and Cgyps1∆ delta cells grown in YNB medium (pH 5.5) and in YNB medium adjusted to pH2.0. Genes involved in carbohydrate and amino acid metabolism, protein folding and stress response pathways were found to be differentially regulated in response to acidic environment in both the strains Agilent one-color experiment,Organism: Yeast ,Agilent-026378 Genotypic designed Custom Candida glabrata 8x15k , Labeling kit: Agilent Quick-Amp labeling
2011-01-10 | E-GEOD-24267 | biostudies-arrayexpress
Project description:Transcriptional time series of Candida glabrata under iron starvation (SD medium without Fe). Wild type and deletion mutants of the iron-related transcription factors Aft1 and Sef1, as well as of the iron uptake transporter Ftr1 as a positive control.
2016-10-05 | GSE84816 | GEO
Project description:Pleiotropic type I interferons (IFNs-I) fulfil multiple protective functions during infectious diseases, but can also exert detrimental effects for the host leading to immunopathology. Here, we report that IFNs-I promote the dysregulation of Fe homeostasis in macrophage populations and in the spleen during systemic infections with the intracellular pathogen Candida glabrata, leading to fungal survival and persistence. By using microarray expression profiling with RNA isolated from mouse spleens upon systemic Candida glabrata infection and subsequent in vitro interaction experiments we show that IFN-I signaling induces global transcriptional downregulation of key players in Fe homeostasis and profoundly alters Fe transport mechanisms within macrophages.
2019-10-29 | GSE134016 | GEO
Project description:To determine the effect of caspofungin (CSP) treatment and/or loss of the SET domain-containing CgSet4 protein on the transcriptional response of log-phase C. glabrata cells. RNA-Seq analysis was conducted on CAA medium-grown log-phase Candida glabrata wild-type (wt) and CgSET4-deleted (Cgset4D) cells in the presence and absence of caspofungin.
2022-09-12 | GSE202654 | GEO
Project description:Total RNA versus genomic DNA hybridization on custom arrays designed for all Candida glabrata genes Total RNA was collected in mid-log phase from Candida glabrata cells grown in rich medium (abbreviated CM, in house recipe). RNA was then converted to cDNA, Cy3-labeled and hybridized competitively against Cy5 labeled genomic DNA from Candida glabrata
2010-07-06 | E-GEOD-22194 | biostudies-arrayexpress