Project description:We report the application of bulk RNA sequencing technology for high-throughput profiling of SARS-CoV-2 infection in the lung of Syrian golden hamsters (Mesocricetus auratus).
Project description:Our aim was to study the gene expression changes associated to the development of non hepatic steatohepatitis from simple steatosis in a model of diet induced obesity in hamster (mesocricetus auratus)
Project description:COVID-19 or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), appeared first in Wuhan, Hubei, China, has emerged as a global health concern claiming millions of lives worldwide. Syrian golden hamster (Mesocricetus auratus) has emerged as a suitable model as when infected by SARS-CoV-2, manifests lung pathology resembling human COVID-19 patients. In this study, we employed a quantitative approach to study the proteomic changes in the SARS-CoV-2-infected lung tissue. The samples were analyzed using Orbitrap Fusion Tribrid mass spectrometer in triplicates and the data acquired was searched against Mesocricetus auratus protein database which resulted in the identification of nearly 2,000 non-redundant proteins. The outcome of this study will facilitate in discovery of potential candidate biomarkers for predicting disease course and warrants their further validation in human patient samples.
Project description:To investigate the mRNA m6A modification profiling in Mesocricetus auratus cells that infected with Senecavirus, we used passage-5 Senecavirus A to infect BSR-T7/5 cells. We then performed m6A MeRIP-seq(GenSeq®️ m6A MeRIP Kit) at at two time points (12hrs or 72 hrs after infection), each time point with three replicates.
