Project description:Transcriptional profiling of Arabidopsis thaliana 12-days old seedlings comparing Col-0 wild type with transgenic plants with altered expression of dual-targetting plastid/mitochondrial organellar RNA-polymerase RPOTmp. Transgenic plants used for experiment were: overexpressor plants obtained by transformation of Col-0 WT plants with genetic constructs created in [Tarasenko et al., 2016] contained catalytic part of RPOTmp enzyme with transit peptides of RPOTm (mitochondrial) and RPOTp (plastid) by agrobacterial transformation; plants with complementation of RPOTmp functions in mitochondria or chloroplasts obtained from transformation of GABI_286E07 rpotmp knockout-mutant plants with genetic constructs created in [Tarasenko et al., 2016]. Goal was to determine the effects of RPOTmp knockout/overexpression on global Arabidopsis thaliana gene expression.
Project description:In order to understand the downstream biological processes and pathways associated to BIR1 induction, we conducted a global transcriptome RNA-Seq assay in Arabidopsis wild-type (WT) and transgenic plants with a dexamethasone (DEX) inducible BIR1-mCherry transgene (ine 9, L9).
Project description:The goal of this study was to compare the transcriptional profile (RNA-seq) of gemin2 Arabidopsis mutant with wild type plants grown under continuous light conditions WT and gemin2 mutant plants were grown for nine days under continuous white light at 22 degrees centigrades and the transcriptional profile of these plants was analyzed using RNA-seq.
Project description:Protein abundance and phosphoproteome profiling of wild-type (WT) as well as quadruple mutant plants deficient in G alpha, G beta, and two out of the three G gamma subunits, in Arabidopsis. WT plants are Col-0 and the quadruple mutant consists ofgpa1-4, agb1-2, agg1-1, and agg2-1 mutants.
Project description:To determine the extent to which the major small RNA pathways functions across the Arabidopsis thaliana genome, small RNA populations from several tissues of wild-type (wt) and mutant plants were amplified by RT-PCR and sequenced using high-throughput 454 sequencing technology. Keywords: small RNAs, high-throughput sequencing
Project description:To gain the possible direct or indirect targets of VaERF73, RNA-Seq was carried out on two biological replicates of wild-type and 3 transgenic Arabidopsis lines mixture (OE1, OE2 and OE3) under normal conditions. The fold change analysis showed that 140 significantly differentially expressed genes were expressed > 2-fold in the transgenic as compared with WT plants. Among these changed genes, 89 genes were up-regulated while 51 genes down-regulated.
Project description:Quantitative proteomic experiments were performed on tryptic digests of proteins purified from the leaves of Arabidopsis thaliana. Wild type plants were compared to bzip28 bzip60 mutant plants. WT and bzip28 bzip60 mutant plants were infected with Drecslera gigantea and compared to non¬-infected control plants
Project description:The goal of this study was to compare the transcriptional profile (RNA-seq) of prmt5 and prmt4a;4b Arabidopsis mutants with their respective wild type plants grown under continous light conditions WT, prmt5 and prmt4a;4b mutant plants were grown together with their respective wild type plants for two weeks under continuous white light at 22 degrees centigrades and the transcriptional profile of these plants was analyzed using RNA-seq.
Project description:To obtain a global view of mRNA uridylation in Arabidopsis, we generated TAIL-seq libraries from WT plants, urt1 and xrn4 single mutants, and urt1 xrn4 double mutant. The TAIL-seq protocol was recently developed to deep-sequence the 3' ends of RNAs (Chang et al., 2014). We generated TAIL-seq libraries from WT plants, urt1 and xrn4 single mutants, and urt1 xrn4 double mutant.
