Project description:We assessed whether organoids maintained the gene expression signatures of their parental healthy tissues and tumors. We performed transcriptomic analysis by bulk RNA sequencing (RNA-seq) across 3 mice, 3 patients and different periods iPSCs. Total RNA of organoids and their parental tissues were extracted at day 7 and day 0, respectively. Total RNA of iPSCs were extracted at day 0, day 1 and day 4. The results show that the organoids are able to replicate the inter-patient heterogeneity, which determines their feasibility on personalized therapy evaluation.
Project description:Interventions: lesion tissues vs. adjacent tissues of colorectal cancer patients:nil
Primary outcome(s): RNA
Study Design: Factorial
Project description:Mex3a is an RNA binding protein of unknown function. To elucidate the contribution of Mex3a to tumoral heterogeneity, Mex3a KO organoids engineered by CRISPR were sequenced in three different conditions. Live organoids (DAPI negative) were sorted in Control, after 2 days of FOLFIRI and after 5 days of treatment. Two WT organoids (parental and a derived clone) and two KO (KO1 and KO2, two independent clones) were used for this experiment.
Project description:This dataset consists of RNA-sequencing of intestinal organoids at rest and after stimulation with the TLR5 ligand flagellin for 4 hours. The goal of this study was to understand the differential patterns of gene expression induced upon stimulation with a TLR ligand in small intestine organoids, colon organoids, and organoids that had been skewed to generate a higher proportion of Paneth cells.
Project description:Retinal organoids samples that derived from human embryonic stem cells were analyzed by single-cell RNA sequencing. Two samples at different differentiation stages (day57 and day 171) were included in this study for cell type comparison.
Project description:RNA transcriptome sequencing analysis was performed in SNU-668 Erastin-resistant cells and SNU-668 parental cells, SNU-484 RSL3-resistant cells and SNU-484 parental cells
Project description:Cortical organoids generated from mESC from a Down Syndrome (DS) mouse model (Ts65Dn) were dissociated for single cell sequencing to understand whether differential gene expression in DS brains affects cell diversity and how the molecular events that control the development of these diverse types of neocortical neurons are affected by the trisomy. These results were generated in parallel to a single cell experiment in forebrain fetal tissue from WT and Ts65Dn mice.