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Psuedouridine sequencing rRNA in Rnmt cKO CD4 T cells

ABSTRACT: Psuedouridine sequencing rRNA in Rnmt cKO CD4 T cells

PROVIDER: PRJNA697920 | ENA |

REPOSITORIES: ENA

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Psuedouridine sequencing rRNA in Rnmt cKO CD4 T cells

Project description:We investigated the role of RNMT in T cells using an Rnmt conditional knockout mouse model. We report that the mRNA cap methyltransferase, RNMT, supports naïve T cell survival and activation-induced proliferation. We demonstrate that RNMT has gene-specific impacts in T cells, selectively regulating expression of terminal polypyrimidine tract (TOP) mRNAs and small RNAs including snoRNAs. To deterine if had any effect on ribosomal RNA modification we carried out pseudoridine sequencing.

2021-05-13 | GSE165790 | GEO

Rnmt cKO versus control activated CD4 T cells

Project description:We aim to find the gene-specific effects of Rnmt KO in mouse CD4 T cells. TMT proteomics datasets were generated to find out which proteins are dependent on RNMT for their expression.

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Rnmt cKO versus control naive CD4 T cells

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Identification of LARP1 targets in naive CD4 T cells [eCLIP]

Project description:We investigated the role of RNMT in T cells using an Rnmt conditional knockout mouse model. We report that the mRNA cap methyltransferase, RNMT, supports naïve T cell survival and activation-induced proliferation. We demonstrate that RNMT has gene-specific impacts in T cells, selectively regulating expression of terminal polypyrimidine tract (TOP) mRNAs which are targets of the m7G-cap binding protein LARP1. These LARP1 eCLIP experiments determine the RNA binding sites of LARP1 in naive CD4 T cells from Rnmt cKO and control mice.

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Rnmt cKO vs control activated CD4 T cell ribo Mega-SEC

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2021-05-13 | PXD023832 | Pride

RNMT regulates the naïve CD4 T cell transcriptome

Project description:We investigated the role of RNMT in T cells using an Rnmt conditional knockout mouse model. We report that the mRNA cap methyltransferase, RNMT, supports naïve T cell survival and activation-induced proliferation. We demonstrate that RNMT has gene-specific impacts in T cells, selectively regulating expression of terminal polypyrimidine tract (TOP) mRNAs which are targets of the m7G-cap binding protein LARP1. These RNAseq experiments investigate the effect of Rnmt cKO on the naïve CD4 T cell transcriptome.

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RNMT regulates the activated CD4 T cell transcriptome and translatome [Ribo-Seq]

Project description:We investigated the role of RNMT in T cells using an Rnmt conditional knockout mouse model. We report that the mRNA cap methyltransferase, RNMT, supports naïve T cell survival and activation-induced proliferation. We demonstrate that RNMT has gene-specific impacts in T cells, selectively regulating expression of terminal polypyrimidine tract (TOP) mRNAs which are targets of the m7G-cap binding protein LARP1. These ribosome footprinting experiments investigate the effect of Rnmt cKO on the activated CD4 T cell transcriptome and translatome.

2021-05-13 | GSE160326 | GEO

low input RNA-sequencing of in vivo activated murine 2D2-WT and 2D2-HDAC1-cKO CD4+ T cells

Project description:An analysis of 2D2-WT and 2D2-HDAC1-cKO mice indicates an important function of HDAC1 in regulating autoimmune diseases, in particular experimental autoimmune encephalomyelitis). The aim of this NGS experiment was to determine the transcirptome profile of in vivo activated 2D2-WT and 2D2-HDAC1-cKO CD4+ T cells in the absence of HDAC1 following MOG/CFA footpad immunization.

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