Project description:Through this comparative proteomic study of equine CD4+ cells 376 membrane proteins could be identified. Several proteins showed changed abundance in horses affected by a spontaneous autoimmune disease (equine recurrent uveitis, ERU). Findings provide novel knowledge about changes in the CD4+ immune cell membrane proteome in a spontaneously and naturally occurring autoimmune disease in horses. The data is highly relevant for veterinary medicine and has proven translational quality for autoimmune uveitis in man.
Project description:We investigated the granulocyte proteome from healthy horses, horses with a spontaneous autoimmune disease (equine recurrent uveitis) and the reaction of granulocytes to different stimuli (IL8, PMA, LPS) on protein level. A total of 2032 proteins describing the whole granulocyte proteome were identified, including 245 proteins (12% of proteome) newly associated to in vivo expression in primary granulocytes (hypothetical proteins). Our studies provide novel information on the granulocyte proteome and contribute to a better understanding of molecular mechanisms involved in activation and recruitment of neutrophils and the role of these cells in modulating and regulating autoimmune diseases. The data presented here is highly relevant for veterinary medicine and has translational quality for spontaneous autoimmune uveitis in man.
Project description:Standardized muscular biopsies of the dorsal compartment of the gluteus medius muscle were performed in 7 horses suffering from equine polysaccharide storage myopathy (PSSM) and 6 sound Norman Cob horses . Gene expression analysis was performed using an equine oligonucleotide microarray which included 384 equine gene probes of the nuclear genome and all the mitochondrial genes.
Project description:Equine recurrent uveitis (ERU) is the only spontaneous model for recurrent autoimmune uveitis in humans, where T cells target retinal proteins. Differences between normal and autoaggressive lymphocytes were identified in this study by analyzing peripheral blood derived lymphocytes (PBL) proteomes from the same case with IRBP-induced uveitis sampled before (day 0), during (day 15) and after uveitic attack (day 23). Relative protein abundances of PBL were investigated in a quantitative, label-free differential proteome analysis in cells that were kept frozen for 14 years since the initial experiment. Quantitative data could be acquired for 2632 proteins at all three time-points. Profound changes (> 2 fold change) in PBL protein abundance were observed when comparing day 0 to 15, representing acute inflammation (234 regulated proteins) and day 0 to 23 (cessation; 382 regulated). Significant differences applied to proteins with functions in integrin signaling during active uveitis, involving “Erk and pi-3 kinase are necessary for collagen binding in corneal epithelia”, “Integrins in angiogenesis” and “Integrin-linked kinase signaling” pathways. In contrast, at cessation of uveitic attack, significantly changed proteins belonged to pathways of “nongenotropic androgen signaling”, “classical complement pathway” and “Amb2 integrin signaling”. Several members of respective pathways were earlier shown to be changed in naturally occurring uveitis, underscoring the significance of these findings here and proofing the value of the induced model in mimicking spontaneous autoimmune uveitis.
Project description:38 horses from 16 diverse breeds and Przewalski's Horse were used to generate a composite CNV map of equine genome. This map was used to detect novel copy number variation in six horses affected with disorder of sexual development (DSD).
Project description:Standardized muscular biopsies of the dorsal compartment of the gluteus medius muscle were performed in 7 horses suffering from equine polysaccharide storage myopathy (PSSM) and 6 sound Norman Cob horses . Gene expression analysis was performed using an equine oligonucleotide microarray which included 384 equine gene probes of the nuclear genome and all the mitochondrial genes. All the samples of PSSM muscles were hybridized against the reference control muscles. This reference was made by pooling together all the mRNA extracted after in vitro transcription from the 6 control muscles of the sound horses. Briefly, the hybridization protocol was adapted from Le Brigand et al. (2006). An open-access long oligonucleotide microarray resource for analysis of the human and mouse transcriptomes. Nucleic Acids Res. 2006 Jul 19;34(12).
Project description:Equine melanocytic neoplasm (EMN) is the common disease in the older grey horse. The purpose of this study was to examine the potential protein throughout EMN stages from fecal proteomic outlining using functional analysis. Fecal samples were collected from rectum of 25 grey horses divided into three groups; normal group without EMN (n=10), mild EMN (n=6) and severe EMN (n=9). From the results, some potential proteins can be used as candidate biomarkers for the monitoring of early EMN, development of EMN, further prevention and treatment.
Project description:An equine immuno-specific oligo microarray platform was designed to evidence differences in gene expression profiles in BAL fluid samples from eight (8) RAO-affected horses, ten (10) IAD-affected horses and seven (7) control animals. An unpaired t test was performed using the software Significant Analysis of Microarrays (SAM). 1763 and 379 genes were found differentially expressed between RAO and IAD horses respectively vs. controls.
