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Lycopene supplementation in serum-free maturation medium improves bovine in vitro embryo development, quality, and modulates embryonic transcriptomic profile

ABSTRACT: Lycopene supplementation in serum-free maturation medium improves bovine in vitro embryo development, quality, and modulates embryonic transcriptomic profile

PROVIDER: PRJNA793780 | ENA |

REPOSITORIES: ENA

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Lycopene supplementation in serum-free maturation medium improves bovine in vitro embryo development, quality, and modulates embryonic transcriptomic profile

Project description:Bovine embryos are typically cultured at reduced oxygen tension to lower the impact of oxidative stress on embryo development. However, oocyte in vitro maturation (IVM) is performed at atmospheric oxygen tension since low oxygen during maturation has a negative impact on oocyte developmental competence. Lycopene, a carotenoid, acts as a powerful antioxidant and may protect the oocyte against oxidative stress during maturation at atmospheric oxygen conditions. Here, we assessed the effect of adding 0.2 μM lycopene (antioxidant), 5 μM menadione (pro-oxidant), and their combination on reactive oxygen species (ROS) generation in mature oocytes and subsequent blastocyst development, quality, and transcriptome in a bovine in vitro model. ROS fluorescent intensity in mature oocytes was significantly lower in the lycopene group, and resulting embryos showed a significantly higher blastocyst rate on day 8 and lower apoptotic cell ratio than all other groups. Transcriptomic analysis disclosed a total of 296 differentially expressed genes (Benjamin-Hochberg adjusted P < 0.05 and ≥ 1 log2 fold change) between lycopene and control groups with pathways associated with cellular function, metabolism, DNA repair, and anti-apoptosis upregulated in lycopene. Lycopene supplementation in serum-free maturation medium neutralizes excess ROS during maturation, enhances blastocyst development and quality, and modulates the transcriptomic landscape.

2022-01-06 | GSE192908 | GEO

Gene expression and pathway analyses following lycopene and fish oil supplementation in men with low-risk prostate cancer

Project description:Animal and epidemiological studies suggest that lycopene and fish oil may modify the risk or delay progression of prostate cancer, however, the molecular mechanisms involved are poorly understood. We examined the effects of these micronutrients on prostate gene expression in a double-blind placebo-controlled randomized clinical trial (Molecular Effects of Nutritional Supplements, MENS). Eighty-four men with low risk prostate cancer were stratified based on self-reported dietary consumption of fish and tomatoes and then randomly assigned to a 3-month intervention of lycopene (intervention B; n = 29) or fish oil (intervention C; n = 27) supplementation or placebo (intervention A; n = 28). Gene expression in morphologically normal prostate tissue was studied at baseline and at 3 months via cDNA microarray analysis. Differential gene expression and pathway analyses were performed to identify genes and pathways modulated by dietary intake of fish or tomato or by lycopene or fish oil supplementation.

2011-06-01 | E-GEOD-27140 | biostudies-arrayexpress

Myoinositol Supplementation of Freezing Medium Improves the Quality-Related Parameters of Dog Sperm.

Project description: Not available

| S-EPMC6940725 | biostudies-literature

Supplementation of biotin to sperm preparation medium enhances fertilizing ability of spermatozoa and improves preimplantation embryo development.

Project description: Not available

| S-EPMC6420554 | biostudies-literature

Growth hormone supplementation reduces aneuploidy and improves the quality of oocytes by activating ERK1/2 pathway in aged mice

Project description:The decline in oocyte quality is a limiting factor of female fertility; however, strategies to maintain the oocyte quality of aged women are not available. In this study, we showed that growth hormone (GH) supplementation in vivo not only alleviated the decline in oocyte number caused by aging, but also improved the quality and developmental potential of aged oocytes. Strikingly, GH supplementation reduced aneuploidy in aged oocytes. Proteomic analysis indicated that the ERK1/2 pathway was involved in the reduction in aneuploidy rate of aged oocytes, as confirmed both in vivo and in vitro. In addition, JAK2 might be involved in the regulation of ERK1/2 by GH in aged oocytes. Collectively, our findings revealed that GH supplementation protects oocytes from aging-related aneuploidy and enhances the quality of aged oocytes, and could be used to improve the outcome of assisted reproduction in aged women.

2023-07-20 | PXD039147 | Pride

Gestational choline supplementation improves cross-generational mood by epigenetic upregulation of Nr3c1

Project description:Gestational choline supplementation improves cross-generational mood by epigenetic upregulation of Nr3c1

| PRJNA328805 | ENA

Nicotinamide mononucleotide supplementation reverses the declining quality of maternally aged oocytes

Project description:Advanced maternal age, defined as 35 years or older, is associated with a decline in both ovarian reserve and oocyte quality, which leads to the female infertility, pregnancy loss, fetal anomalies, stillbirth, and obstetric complications. At present, the effective approaches to counteract the maternal age-related decay of oocyte quality are still not fully determined. Here, we report that in vivo supplementation of nicotinamide mononucleotide (NMN) efficaciously ameliorates the quality of oocytes from naturally aged mice by recovering nicotinamide adenine dinucleotide (NAD + ) levels in oocytes. NMN supplementation increases the number of antral follicles, ovulated oocytes and matured oocytes from aged mice. Specifically, NMN supplementation maintains the normal spindle/chromosome structure and dynamics of cortical granule component ovastacin to ensure the meiotic competency and fertilization ability of aged oocytes. Moreover, single cell transcriptome analysis shows that the beneficial effect of NMN on the aged oocytes is mediated by the restoration of the mitochondrial function, thereby reducing the accumulated ROS to suppress the occurrence of apoptosis. To sum up, our data reveal that supplementation of NMN is a feasible approach to prevent oocyte quality from advanced maternal age-related deterioration, contributing to improve the reproductive outcome of aged women and the assisted reproductive technology.

2023-07-01 | GSE153920 | GEO

Supplementation of fetal bovine serum increased the quality of <i>in vitro</i> fertilized porcine embryo.

Project description: Not available

| S-EPMC8757674 | biostudies-literature

Embryo Aggregation in Pig Improves Cloning Efficiency and Embryo Quality.

Project description: Not available

| S-EPMC4760708 | biostudies-literature

Gene expression in human keratinocytes cultivated with serum-free medium and serum containing medium

Project description:The objective of this study was thus to develop ab initio that chemically defined serum-free medium. We used a statistical design of experiment (DOE) approach to screen a large number of candidate factors, for both their potential individual and synergetic effects, as to identify a formulation which was then tested extensively. In this study, we present the properties of keratinocyte cultivated with serum-Free medium (Surge SFM) and complemented DME-Ham medium (with serum).

2021-09-08 | GSE183594 | GEO

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