Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Liver_Micro_UT

ABSTRACT: Liver Microsomal Fraction - UT

INSTRUMENT(S): LCQ DECA XP

ORGANISM(S): Mus Musculus

SUBMITTER: Andrew Emili

PROVIDER: PAe000376 | PeptideAtlas |

REPOSITORIES: PeptideAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	PAe000376_4966_search.params.tar.gz	Other
	PAe000376_4966_searchdatabase_tandem.fasta	Fasta
	PAe000376_4966_tandem.params	Other
	PAe000376_RAW_201104191034.tar.gz	Other
	PAe000376_README	Other

Items per page:

1 - 5 of 11

Similar Datasets

Identification of the UT-A1 Urea Channel Interactome

Project description:The urea channel Slc14a2 (or UT-A1) mediates vasopressin-regulated urea transport across the inner medullary collecting duct (IMCD). Previously, UT-A1 was found to present in a high molecular weight complex, suggesting UT-A1 is involved in certain protein-protein interactions. The present study sought to identify the proteins that interact with UT-A1 in this complex for a better understanding of how UT-A1 is regulated. Rat IMCD suspensions were treated with or without V2 receptor agonist, dDAVP, followed by in-cell crosslinking using BSOCOES and detergent solubilization. Immunoprecipitation using Dynabeads coated with UT-A1 specific antibody successfully pulled down the UT-A1 proteins. In-gel digestion protocol was carried out to prepare samples for liquid chromatographic mass spectrometry analysis of tryptic peptides using a Velos-Orbitrap mass spectrometer. The peptides passing stringent spectral quality thresholds were quantified (label-free) to identify those with (UTA-1 antibody/preimmune IgG) >4. A total of 128 UT-A1 interacting proteins were identified. Gene Ontology analysis maps the distribution of these proteins throughout major cell compartments: endoplasmic reticulum, Golgi, endosomes, cytosol and plasma membrane. Among them are four protein kinases (Cdc42bpb, Phkb, Camk2d, Mtor) that play roles in vasopressin-regulated phosphorylation of UT-A1. Non-label quantification was also performed to determine the stoichiometry of UT-A3 with UT-A1, the result does not support an oligomeric complex formation of UT-A1/A3. In conclusion, we have provided a refined list of UT-A1 binding proteins which can be useful for further analysis of the vasopressin signaling pathway in regulation of UT-A1 in IMCD.

2019-11-08 | PXD003496 | Pride

A_niger_microsome - Proteomic analysis of the secretory response of Aspergillus niger to D-maltose and D-xylose

Project description:Shotgun proteomics of microsomal enriched fraction 2 h after addition 0f 10mM xylose to sorbitol grown mycelium

2012-05-17 | PRD000334 | Pride

Fenbendazole Met ID_in vitro_cross-species_FBMN

Project description:Fenbendazole Met ID by an integrative approach using feature-based molecular networking of cross-species in vitro liver microsomal and hepatocellular incubation.

2024-08-08 | MSV000095554 | MassIVE

Placenta_Micro_UT

Project description:Placenta Microsomal Fraction - UT

| PAe000381 | PeptideAtlas

Lung_Micro_UT

Project description:Lung Microsomal Fraction - UT

| PAe000363 | PeptideAtlas

Heart_Micro_UT

Project description:Heart Microsomal Fraction - UT

| PAe000354 | PeptideAtlas

Brain_Micro_UT

Project description:Brain Microsomal Fraction - UT

| PAe000370 | PeptideAtlas

Kidney_Micro_UT

Project description:Kidney Microsomal Fraction - UT

| PAe000368 | PeptideAtlas

Quantitative LC-MS/MS Proteomics of human liver microsomes (healthy controls and normal-tumour from cancer patients)

Project description:We report quantification of proteins in human liver microsomal samples from 15 healthy volunteers and 18 patients with cancer in the liver (mainly, colorectal cancer liver metastasis). These data can be used in physiologically based pharmacokinetic models to predict appropriate drug doses in patients with cancer in their liver, especially colorectal cancer liver metastasis.

2023-05-10 | PXD038776 | Pride

Autoantibodies secreted by TLR7-stimulated B cell subsets from mice with primary Sjogren's syndrome

Project description:Spleens (n = 2-3) were harvested from mice with primary Sjogren's syndrome (NOD.B10) and cells were sort-purified. Follicular B cells (FO) (6 million), Marginal zone B cells (4.5 million) and age-associated B cells (500,000) were sorted. Cells were cultured in 200 uL of complete RPMI media containing 62.5 ng/mL of imiquimod, a TLR7 agonist. Cells were cultured for 6 days, and the supernatants were harvested and stored at -20 C. Samples were shipped to UT Southwestern for autoantigen array analysis.

2023-10-24 | GSE236255 | GEO