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Proteomic characterisation of microtubule-dependent modulation of adhesion complexes

ABSTRACT: Investigating the effect of microtubule disruption by nocodazole treatment on isolated adhesion complexes.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Testis, Primary Cell, Fibroblast

SUBMITTER: Daniel Ng

LAB HEAD: Martin James Humphries

PROVIDER: PXD001183 | Pride | 2015-01-30

REPOSITORIES: Pride

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Microtubule-dependent modulation of adhesion complex composition.

Ng Daniel H J DH Humphries Jonathan D JD Byron Adam A Millon-Frémillon Angélique A Humphries Martin J MJ

PloS one 20141219 12

The microtubule network regulates the turnover of integrin-containing adhesion complexes to stimulate cell migration. Disruption of the microtubule network results in an enlargement of adhesion complex size due to increased RhoA-stimulated actomyosin contractility, and inhibition of adhesion complex turnover; however, the microtubule-dependent changes in adhesion complex composition have not been studied in a global, unbiased manner. Here we used label-free quantitative mass spectrometry-based p ...[more]

PMID: 25526367

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