Proteomics

Dataset Information

0

Franco-Swiss HPP project (Chromosomes 2 and 14)


ABSTRACT: The dataset is a concatenation of nanoLC-MS/MS analyses performed on different sample types/sample preparations of human proteins on various instrumental platforms in the laboratory. A franco-swiss collaboration to identify missing human proteins on chromosomes 2 and 14 in the frame of the Human Proteome Project, set up a bioinformatic screen over all datasets accumulated in the laboratory in order to detect proteins classified as “missing” by NextProt.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Spleen, Urine, Blood Cell, Cell Suspension Culture, Blood Plasma, Leukocyte, Cerebrospinal Fluid, Permanent Cell Line Cell, Hela Cell, Erythrocyte, Primary Cell, Cell Culture, Fibroblast, Jurkat Cell

DISEASE(S): Brain Glioblastoma Multiforme,Lymphoma

SUBMITTER: Emmanuelle Mouton Barbosa  

LAB HEAD: Odile Bruley Schiltz

PROVIDER: PXD002131 | Pride | 2015-07-17

REPOSITORIES: Pride

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Publications


In the framework of the C-HPP, our Franco-Swiss consortium has adopted chromosomes 2 and 14, coding for a total of 382 missing proteins (proteins for which evidence is lacking at protein level). Over the last 4 years, the French proteomics infrastructure has collected high-quality data sets from 40 human samples, including a series of rarely studied cell lines, tissue types, and sample preparations. Here we described a step-by-step strategy based on the use of bioinformatics screening and subseq  ...[more]

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