Proteomics

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Multi-omics toxicity profiling of engineered nanomaterials


ABSTRACT: This study aims to implement methods for toxicological profiling of engineered nanomaterials using toxicogenomics, proteomics, and metabolomics along with computational analyses. For all three omics layers the human cell lines A549 (lung epithelial cells) and THP1 (monocytes) were separately exposed to the nanomaterials TiO2 NM104, MWNCT NM401, and Ag NM300k. Proteomics and metabolomics samples have been performed as biological triplicates and were taken after 0, 6, 12, 24, and 48 hours. To assess ecotoxic effects C. elegans worms were grown in soil treated with NM300k. Ecotox samples were taken only at 0 and 24 hours. Integrating all three omics layers will enable the identification of (novel) ENM specific modes of action (MoA).

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Strigamia maritima   Cricetulus griseus   Pyrococcus furiosus   Chlamydomonas reinhardtii   Gammarus pulex   Crassostrea gigas   Trypanosoma cruzi   Neurospora crassa   Bartonella quintana   Mycoplasma gallisepticum S6   Vibrio cholerae O1 biovar El Tor str. N16961   Oreochromis mossambicus   environmental samples   Allexis   Francisella tularensis   Austrolebias   Oryctolagus cuniculus algirus   Anopheles culicifacies B   Manica rubida   Clostridium butyricum E4 str. BoNT E BL5262   Brassica oleracea var. italica   Olavius algarvensis Gamma 3 endosymbiont   Drosophila melanogaster   Streptomyces avidinii   Pseudomonas putida BIRD-1   Bordetella pertussis Tohama I   Glia   Nicotiana benthamiana   Human immunodeficiency virus 1   Burkholderia cenocepacia   Tuber melanosporum Mel28   Tuber indicum   Mycobacterium tuberculosis SP10   Bubalus bubalis   Pyricularia oryzae   Listeria monocytogenes   Taenia hydatigena   Aspergillus niger   Francisella tularensis subsp. holarctica FSC200   Blumeria hordei DH14   Medicago sativa   Acipenser transmontanus   Candida tropicalis   Sarcoptes scabiei   Streptococcus equi subsp. equi 4047   Clostridium autoethanogenum   Flavobacterium columnare   Lactobacillus   Olavius algarvensis Gamma 1 endosymbiont   Leishmania infantum JPCM5   Phytophthora infestans   Aspergillus aff. niger A2   Entamoeba histolytica HM-1:IMSS-A   Mariprofundus ferrooxydans   Phaeobacter sp. MED193   Taeniopygia guttata   Paucilactobacillus suebicus   environmental samples   Thalassiosira pseudonana CCMP1335   Physcomitrium patens   Leishmania donovani   Larimichthys crocea   Cyclopes didactylus   Bacillus cytotoxicus NVH 391-98   Cryptococcus neoformans var. grubii H99   Naja naja   Capra hircus   Severe acute respiratory syndrome coronavirus 2   Trypanosoma brucei brucei   Solanum tuberosum   Beauveria bassiana ARSEF 2860   Muvirus mu   Syntrophomonas wolfei   Apteronotus leptorhynchus   Oryza sativa Indica Group   Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821   Microcystis aeruginosa   Aves   uncultured Phormidium sp.   Bothrops jararacussu   Coxiella burnetii RSA 493   Bothrops brazili   Malassezia sympodialis ATCC 42132   Solanum lycopersicum   Tuber aestivum   Fasciola hepatica   Sorghum bicolor   Histomonas meleagridis   Bacillus mycoides DSM 2048   Ovis aries   Saccharomyces cerevisiae EC1118   Pogonomyrmex rugosus   Giardia lamblia ATCC 50803   Danio rerio   Glycine max   Porphyromonas gingivalis W83   unclassified Candidatus Omnitrophica   Naja mandalayensis   Nicotiana tabacum   Lithobius forficatus   Burkholderia pseudomallei 1026b   Heloderma suspectum suspectum   Quercus ilex   Euzebya tangerina   Parasynechococcus marenigrum WH 8102   Synechococcus phage syn9   Scheffersomyces stipitis   Strongylocentrotus purpuratus   Plasmodium yoelii yoelii   Listeria monocytogenes EGD-e   Mengo virus   Saccostrea glomerata   Viruses   Thermoplasma acidophilum DSM 1728   Mycobacterium tuberculosis KZN 1435   Epstein-barr virus strain p3hr-1   Campylobacter jejuni   Chenopodium quinoa   Giardia intestinalis assemblage A   Haptophyta   Trypanosoma brucei brucei TREU927   Zea mays   Clostridioides difficile 630   Olavius algarvensis spirochete endosymbiont   Botrytis cinerea   Mycobacterium tuberculosis SP7   Neisseria meningitidis MC58   Ascaris suum   Emiliania   Brucella abortus   Spiroplasma melliferum KC3   Vitis   Vibrio anguillarum   Granulicella sp.   Paramoeba perurans   Bacillus subtilis   Bursaphelenchus xylophilus   Piaractus mesopotamicus   Kluyveromyces marxianus   Penaeus vannamei   Haemophilus influenzae   Meleagris gallopavo   Fraxinus nigra   Dioscorea alata   uncultured marine microorganism   Latilactobacillus curvatus   Mus musculus   Apis mellifera ligustica   Phaseolus vulgaris   biofilm metagenome   Mytilus edulis/galloprovincialis MCLGV-2007   Paenibacillus sp.   Trichoderma harzianum CBS 226.95   Vibrio alginolyticus   Arabidopsis thaliana   Trifolium pratense subsp. pratense   Helicobacter pylori J99   Cavia porcellus   Channa striata   Homo   Pseudomonas stutzeri   Cupriavidus metallidurans   Eukaryota   Siphoviridae   Salmo salar   Nephila clavipes   Bacillus subtilis subsp. subtilis str. 168   Bothrops insularis   Olavius algarvensis sulfate-reducing endosymbiont   Oreochromis niloticus niloticus   Shigella dysenteriae   Ruminiclostridium papyrosolvens DSM 2782   Morchella importuna   Rattus rattus   Ictidomys tridecemlineatus   Triticum aestivum   Tarenaya hassleriana   Fusarium graminearum GZ3639   Bradyrhizobium japonicum   Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759   Struthio camelus   Cleome angustifolia   Tetrahymena thermophila   Pedobacter sp.   Staphylococcus sp. 1   Peniophora incarnata   Vipera berus berus   Macaca mulatta   Streptococcus pneumoniae TIGR4   Mycobacterium tuberculosis M24   Desulfovibrio sp. G11   Tropilaelaps mercedesae   Catenibacterium   Mycolicibacterium smegmatis   Toxoplasma gondii   Dasypogon diadema   Enterococcus faecalis   Scolopendra morsitans   Saccharomyces cerevisiae W303   Ustilago maydis   Escherichia coli O157:NM   Spondylus gaederopus   Gammarus fossarum   Mustela putorius furo   Homo sapiens   Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841   Roseburia   Human herpesvirus 2 strain 186   Emiliania huxleyi   Rhodopseudomonas palustris   Equus caballus   Saccharopolyspora erythraea   Clostridium phage c-st   Lens culinaris   Mytilus sp. CHGF4   Bemisia tabaci   Bradyrhizobium diazoefficiens USDA 110   Capsicum annuum   Sorangium cellulosum So ce56   Felis catus   Populus tremula x Populus alba   Burkholderia ubonensis MSMB22   Phytophthora sojae   Trypanosoma   Betaproteobacteria bacterium RIFCSPLOWO2_02_64_14   Daphnia pulex   Thalassiosira cf. oceanica   Mycobacterium marinum ATCC BAA-535   Eremothecium gossypii   Leishmania donovani Ld 2001   Mollivirus sibericum   Heterorhabditis megidis   Vitis vinifera   Pseudomonas aeruginosa PAO1H2O   Cyclotella cryptica   Sus scrofa   Gynandropsis gynandra   Helicobacter pylori 26695   Enterococcus mundtii CRL35   Spirochaeta sp. ELBA   Streptococcus pyogenes   Tradescantia spathacea   Bartonella quintana JK 31   Desulforamulus reducens MI-1   Aerococcus urinae NBRC 15544 = CCUG 36881   Aggregatibacter actinomycetemcomitans   Brassica napus   Hebomoia glaucippe   Theobroma cacao   Ficus carica   Acanthoscurria geniculata   Crassostrea hongkongensis   Elaeis guineensis   Schizosaccharomyces pombe 927   Mycoplasma pneumoniae M129-B7   Onchocerca volvulus   Mytilus edulis   Piper nigrum   Francisella tularensis subsp. holarctica LVS   [Ruminococcus] gnavus   Burkholderia sp. 2332   Candida albicans   Coffea canephora   Schizosaccharomyces pombe OY26   Campylobacter fetus subsp. fetus 82-40   Beta vulgaris subsp. vulgaris   Paracoccus denitrificans PD1222   Capsaspora owczarzaki   Giardia intestinalis   Rattus norvegicus   Botrytis cinerea B05.10   Acinetobacter baumannii AB307-0294   Bothrops moojeni   Dirofilaria immitis   Globicatella sanguinis   Dinoroseobacter shibae DFL 12 = DSM 16493   Mycobacteroides abscessus   Chlamydia trachomatis 434/Bu   Acidithiobacillus ferrooxidans ATCC 53993   Naja sagittifera   Ganoderma lingzhi   Geobacter sulfurreducens   Escherichia coli   Amphibalanus amphitrite   Citrus maxima   prokaryotic environmental samples   Trichechus manatus   Salmo sp.   Vitis riparia   Steinernema carpocapsae   environmental samples   Dioscorea oppositifolia   Pachysolen tannophilus NRRL Y-2460   Hepatitis E virus type 1   Shigella sonnei 53G   Quercus suber   Euprymna scolopes   Shigella flexneri 2a str. 2457T   Anopheles culicifacies A   Bacillus toyonensis BCT-7112   Ceratopteris richardii   Aspergillus flavus NRRL3357   Actinomycetia   Puccinellia tenuiflora   human metagenome   Crithidia fasciculata   Ruminiclostridium cellulolyticum H10   Mycobacterium tuberculosis H37Rv   Clostridioides difficile R20291   Halobacterium salinarum   Palaemonetes sp. common grass shrimp associated circular virus   Lactobacillus johnsonii N6.2   Alternaria tomato   Synechocystis sp. PCC 6803   Listeria phage A118   Tapirus terrestris   Podospora anserina S mat+   Plasmodium knowlesi strain H   Sphingomonas sp. Leaf257   Dialister   Candidatus Accumulibacter phosphatis   Coprococcus eutactus   Trichoderma reesei RUT C-30   Aspergillus niger NRRL3   Noccaea caerulescens   Olavius algarvensis   Bacillus thuringiensis serovar berliner ATCC 10792   Ursus arctos arctos   Pseudomonas aeruginosa PA14   Quercus petraea   H1N1 subtype   Helianthus annuus   Dendroaspis angusticeps   Human alphaherpesvirus 1 strain 17   Synechococcus elongatus   (Vitis vinifera x Vitis berlandieri) x Vitis berlandieri   Gossypium hirsutum   Cervus elaphus elaphus   Stenotrophomonas maltophilia   Calicophoron daubneyi   environmental samples   Oncorhynchus tshawytscha   Brugia malayi   Homo sapiens subsp. 'Denisova'   Acanthamoeba castellanii   Mylodon darwinii   Schizosaccharomyces pombe 972h-   Synechococcus cyanophage syn   Sedum plumbizincicola   Bovine leukemia virus   Trichoderma sp. 1 YW-2011   Hordeum vulgare   Phytophthora capsici LT1534   Leishmania major   Bacillus subtilis subsp. spizizenii ATCC 6633 = JCM 2499   Quercus robur   Medicago truncatula   Camelus dromedarius   Streptococcus agalactiae FSL S3-026   Scutigera coleoptrata   rhizosphere soil bacteria ensemble   Trichomonas gallinae   Acinetobacter baumannii D1279779   Paramecium tetraurelia   Passiflora edulis   Fragaria x ananassa   Dentex dentex   Candidatus Nitrospira inopinata   Vicia faba   Eulimnogammarus cyaneus   Citrus sinensis   Leishmania mexicana   Medicago truncatula var. truncatula   Aspergillus nidulans TN02A3   Escherichia coli O157:H7 str. TW14359   Parhyale hawaiensis   Moraxella catarrhalis   Riftia pachyptila   Chlorocebus sabaeus   Clostridium saccharoperbutylacetonicum   Bodo saltans   Luteibacter   Francisella tularensis subsp. holarctica   Drosophila   Hendra virus horse/Australia/Hendra/1994   Ricinus communis   Mesocricetus auratus   Arachis hypogaea   Amaranthus tricolor   Entamoeba histolytica   Taenia solium   Dictyostelium discoideum AX4   Candidatus Endoriftia persephone   Rhizobium cf. leguminosarum   Rhizophagus irregularis   Coffea eugenioides   Escherichia coli K-12   Schistosoma mansoni   Lotus japonicus   Melioribacter roseus P3M-2   Bacteria   Plasmodium falciparum 3D7   Chlorocebus aethiops   Fraxinus mandshurica   Human immunodeficiency virus   Olavius algarvensis Delta 1 endosymbiont   [Candida] glabrata   Scophthalmus maximus   Bothrops leucurus   Pectobacterium carotovorum   anaerobic digester metagenome   Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10   Gallus gallus   Bacteroides caccae   Lactococcus lactis subsp. cremoris UC509.9   Gorilla gorilla   Faecalibacterium prausnitzii   Novosphingobium nitrogenifigens   Mycobacterium tuberculosis SP22   Dokdonia sp. MED134   Trichoderma reesei QM9414   Brucella   Macaca fascicularis   Chlamydia trachomatis D/UW-3/CX   Roseobacter sp. MED193   Marchantia polymorpha subsp. ruderalis   Streptococcus pyogenes ABC020006030   Cytomegalovirus   Streptococcus thermophilus LMD-9   Malus domestica   Lagomorpha   Cladocopium goreaui   Sinorhizobium meliloti BL225C   Streptococcus pneumoniae D39   Nelumbo nucifera   Granulicatella adiacens ATCC 49175   Riptortus pedestris   Coffea arabica   Streptomyces coelicolor A3(2)   Cyanophora paradoxa   Tuber albidum   Aspergillus flavus   Picea likiangensis var. rubescens   Neptuniibacter caesariensis   Spiroplasma melliferum   Ophrys garganica   hybrid   Sophora alopecuroides   unidentified   Sus scrofa domesticus   Avena strigosa   Staphylococcus haemolyticus   unclassified Marseilleviridae   Octopodiformes   Boehmeria nivea   Toxoplasma gondii type III   Dendroaspis polylepis polylepis   Physcomitrella   Exiguobacterium sp. SH31   Equus asinus   Ensifer adhaerens   Delonix regia   Phytophthora capsici   Trypanosoma cruzi strain Esmeraldo   Candidatus Pelagibacter ubique   Staphylococcus aureus   Chromatiales bacterium OalgGamma1   Mycobacterium tuberculosis SP13   Andreprevotia sp. IGB-42   Corynebacterium glutamicum ATCC 13032   Thermochaetoides thermophila   Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044   Apostichopus japonicus   Labeo rohita   Brucella melitensis   Hyalella azteca   Daktulosphaira vitifoliae   Mycoplasma pneumoniae M129   Bombyx mori   Borreliella burgdorferi B31   Russula atropurpurea   gut metagenome   Mycoplasmopsis agalactiae   Thermococcus gammatolerans EJ3   Brachyspiraceae   Thermochaetoides thermophila DSM 1495   Plasmodium sp. pBT9   Sphingobium chlorophenolicum   Deltaproteobacteria bacterium RIFCSPLOWO2_12_FULL_50_11   Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344   Legionella pneumophila subsp. pneumophila str. Philadelphia 1   Dictyostelium discoideum   Pseudotsuga menziesii var. menziesii   Burkholderia cenocepacia K56-2Valvano   Hippopotamus amphibius   Brassica oleracea var. botrytis   Tequatrovirus T4   Mycolicibacterium smegmatis MC2 155   Anopheles stephensi   Picea abies   Pyricularia oryzae B157   Lactococcus phage   Butyrivibrio fibrisolvens   Bothrops neuwiedi   Human alphaherpesvirus 1 strain KOS   metagenomes   Acinetobacter baumannii ACICU   Strigomonas culicis   Chlorella vulgaris   Populus trichocarpa   Olavius algarvensis Delta 4 endosymbiont   Nematostella vectensis   Oryza sativa   Oryctolagus cuniculus   Ophrys x arachnitiformis subsp. archipelagi   Echinococcus granulosus   Enterobacter cloacae   mixed culture metagenome   Chlorella sp. SAG 211-18   Ctenopharyngodon idella   Coxiella burnetii   Bos taurus   Lentinus crinitus   Salmonella enterica subsp. enterica serovar Typhimurium str. SDT1291   Phytophthora pisi   Bicyclus anynana   Angiostrongylus cantonensis   albimanus section   Leishmania major strain Friedlin   Klebsiella pneumoniae   Pseudomonas aeruginosa PAO1   Aedes aegypti aegypti   Orycteropus afer   Bacillus pseudomycoides DSM 12442   Canis lupus familiaris   Deinococcus radiodurans R1   Toxoplasma gondii ME49   Breviolum minutum   Citrobacter rodentium   Candidatus Accumulibacter   Trypanosoma brucei   Xanthomonas oryzae pv. oryzae PXO99A   Streptococcus dysgalactiae subsp. equisimilis SK1249   Pseudomonas pseudoalcaligenes CECT 5344   Human herpesvirus 5 strain AD169   Vibrio parahaemolyticus   Brassica oleracea var. gemmifera   Pteropus alecto   Cecropia peltata   Synechocystis sp. PCC 6803 substr. Kazusa   Alteromonadales   Lysiphlebus fabarum   Schizosaccharomyces pombe DM3650   Synechococcus phage S-SSM7   Hydra vulgaris   Human alphaherpesvirus 1   Clavibacter michiganensis subsp. nebraskensis NCPPB 2581   Escherichia coli O157:H7   Zymomonas mobilis   soil bacterium TS1114R   Bombus terrestris audax   Streptococcus pneumoniae R6   Oryctolagus sp. 'Rabbit_OD'   Pseudomonas putida F1   Ostreococcus tauri   Pan troglodytes   Capsicum annuum var. annuum   Potentilla saundersiana   Caenorhabditis elegans   Cervus nippon nippon   Canis lupus x Canis lupus familiaris   Strawberry leafy fruit phytoplasma   Colinus virginianus   Xenopus laevis   Panicum virgatum   Tuber magnatum   Mycobacterium avium subsp. paratuberculosis K-10   Planococcus halocryophilus Or1   Pyrococcus abyssi   Pyricularia oryzae P131   Anas platyrhynchos   Oryctolagus cuniculus cuniculus   Rhodococcus jostii RHA1   Teleogryllus oceanicus   Ophrys sphegodes   Acyrthosiphon pisum   Selaginella moellendorffii   Spirometra erinaceieuropaei   Durusdinium trenchii   Latrodectus hesperus   Herpes simplex virus unknown type   Saccharomyces cerevisiae   Flavobacterium sp. 316   Lactococcus lactis subsp. cremoris MG1363   Mucilaginibacter   Rheum palmatum   Saccharum hybrid cultivar   Desulfovibrio ferrophilus   Apis mellifera   Rhizobium sp. Leaf68   Archaea   Clostridioides difficile ATCC 43255   Bothrops cotiara   Acanthoscurria gomesiana   Coronavirus PREDICT CoV-2   Bacillus mycoides NBRC 101238 = DSM 11821   Dactylorhiza   Candida albicans SC5314   Schizophyllum commune H4-8   Populus yunnanensis   Mycobacterium tuberculosis SP1   Leishmania braziliensis MHOM/BR/75/M2904   Gammarus roeselii   Lingula anatina   Influenza A virus (H1N1)   Pseudomonas aeruginosa   Callinectes sapidus   Streptococcus pneumoniae SP14-BS69   Mycobacterium tuberculosis   Sciuridae   Cryptococcus neoformans var. grubii H99W   Pseudomonas syringae   Cryptochiton stelleri   Cupiennius salei   Oncorhynchus mykiss   Naja ashei   Armoracia rusticana   Plasmodium falciparum   Pseudomonas putida KT2440   Heterosigma akashiwo   Acinetobacter baumannii   Pseudomonas fluorescens SBW25   Streptomyces coelicolor   Photobacterium profundum SS9   Paracoccidioides brasiliensis Pb18   Oryzias latipes   Daphnia magna   Cercopithecidae   Craterostigmus tasmanianus   Candidatus Thioglobus autotrophicus   Lacticaseibacillus rhamnosus GG   Catalpa fargesii   Schizosaccharomyces pombe   Bothrops jararaca   Bacillus cereus ATCC 14579   Oryza sativa Japonica Group   Priestia megaterium DSM 319   Ictalurid herpesvirus 1  

TISSUE(S): Monocyte, Epithelial Cell, Cell Culture

SUBMITTER: Marc Rurik  

LAB HEAD: Christian Huber

PROVIDER: PXD002401 | Pride | 2016-07-25

REPOSITORIES: Pride

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