Proteomics

Dataset Information

79

Phosphoproteomic studies of role of LAR protein tyrosine phosphatase in mouse embryonic fibroblasts


ABSTRACT: To gain insights into the signalling pathways regulated by Leukocyte common antigen-related protein (LAR), including those that are PDGF-dependent, we have carried out the first systematic analysis of LAR-regulated signal transduction using SILAC-based quantitative proteomic and phosphoproteomic techniques. We have analysed differential phosphorylation between wild-type mouse embryo fibroblasts (MEFs) or MEFS in which the LAR cytoplasmic phosphatase domains had been deleted (LARΔP).

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus musculus  

TISSUE(S): Cell Culture

DISEASE(S): Disease Free

SUBMITTER: Debbie Cunningham  

LAB HEAD: Debbie L Cunningham

PROVIDER: PXD002545 | Pride | 2016-04-15

REPOSITORIES: Pride

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Publications

Regulation of Platelet Derived Growth Factor Signaling by Leukocyte Common Antigen-related (LAR) Protein Tyrosine Phosphatase: A Quantitative Phosphoproteomics Study.

Sarhan Adil R AR   Patel Trushar R TR   Creese Andrew J AJ   Tomlinson Michael G MG   Hellberg Carina C   Heath John K JK   Hotchin Neil A NA   Cunningham Debbie L DL  

Molecular & cellular proteomics : MCP 20160413 6


Intracellular signaling pathways are reliant on protein phosphorylation events that are controlled by a balance of kinase and phosphatase activity. Although kinases have been extensively studied, the role of phosphatases in controlling specific cell signaling pathways has been less so. Leukocyte common antigen-related protein (LAR) is a member of the LAR subfamily of receptor-like protein tyrosine phosphatases (RPTPs). LAR is known to regulate the activity of a number of receptor tyrosine kinase  ...[more]

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