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Salmon larvae proteomics - Sequential protein extraction as an efficient method for improved proteome coverage in larvae of Atlantic salmon (Salmo salar)

ABSTRACT: Application of a solubility-based sequential protein extraction method to improve proteome coverage in whole fish

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Salmo Salar (atlantic Salmon)

TISSUE(S): Whole Body

SUBMITTER: Richard Wilson

LAB HEAD: Dr Richard Wilson

PROVIDER: PXD003366 | Pride | 2016-06-13

REPOSITORIES: Pride

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Sequential protein extraction as an efficient method for improved proteome coverage in larvae of Atlantic salmon (Salmo salar).

Nuez-Ortín Waldo G WG Carter Chris G CG Nichols Peter D PD Wilson Richard R

Proteomics 20160623 14

Understanding diet- and environmentally induced physiological changes in fish larvae is a major goal for the aquaculture industry. Proteomic analysis of whole fish larvae comprising multiple tissues offers considerable potential but is challenging due to the very large dynamic range of protein abundance. To extend the coverage of the larval phase of the Atlantic salmon (Salmo salar) proteome, we applied a two-step sequential extraction (SE) method, based on differential protein solubility, using ...[more]

PMID: 27272914

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