Proteomics

Dataset Information

86

Comprehensive Immunoproteome of Mycoplasma bovis


ABSTRACT: This study was aimed to elucidate a global antigenic profile of Mycoplasma bovis (M. bovis) with immunoproteomics, immunoinformatics, and gene expression approaches. The extracts of whole-cell proteins and TX-114 membrane fraction of a Chinese strain M. bovis HB0801 were separated with two dimensional gel electrophoresis (2-DE) and proteins reacting with antisera to M. bovis from experimentally infected calves were detected by MALDI-TOF MS.

INSTRUMENT(S): 4800 Proteomics Analyzer

ORGANISM(S): Bos taurus   Mycoplasma bovis HB0801  

TISSUE(S): Blood Serum

DISEASE(S): Not Available

SUBMITTER: Farhan Anwar  

LAB HEAD: Aizhen Guo

PROVIDER: PXD003479 | Pride | 2016-06-13

REPOSITORIES: pride

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Publications

Immunoproteomic identification of MbovP579, a promising diagnostic biomarker for serological detection of Mycoplasma bovis infection.

Khan Farhan Anwar FA   Faisal Muhammad M   Chao Jin J   Liu Kai K   Chen Xi X   Zhao Gang G   Menghwar Harish H   Zhang Hui H   Zhu Xifang X   Rasheed Muhammad Asif MA   He Chenfei C   Hu Changmin C   Chen Yingyu Y   Baranowski Eric E   Chen Huanchun H   Guo Aizhen A  

Oncotarget 20160601 26


A lack of knowledge regarding the antigenic properties of Mycoplasma bovis proteins prevents the effective control of bovine infections using immunological approaches. In this study, we detected and characterized a specific and sensitive M. bovis diagnostic biomarker. After M. bovis total proteins and membrane fractions were separated with two dimensional gel electrophoresis, proteins reacting with antiserawere detected using MALDI-TOF MS. Thirty-nine proteins were identified, 32 of which were p  ...[more]

Publication: 1/2

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