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Dynamic of the Grb2 interactome in primary cells by DIA-MS

ABSTRACT: We applied AP-SWATH to quantify Grb2 interaction dynamics in primary T cells with a high level of reproducibility and accuracy. We concluded that AP-SWATH is a robust approach to quantify signaling dynamics in primary cells and could be further expanded to additional hub proteins using a range of cell and tissues types.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spleen, T Cell, Lymph Node, Thymus

SUBMITTER: Etienne Caron

LAB HEAD: Matthias Gstaiger

PROVIDER: PXD003972 | Pride | 2017-03-09

REPOSITORIES: Pride

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Precise Temporal Profiling of Signaling Complexes in Primary Cells Using SWATH Mass Spectrometry.

Caron Etienne E Roncagalli Romain R Hase Takeshi T Wolski Witold E WE Choi Meena M Menoita Marisa G MG Durand Stephane S García-Blesa Antonio A Fierro-Monti Ivo I Sajic Tatjana T Heusel Moritz M Weiss Tobias T Malissen Marie M Schlapbach Ralph R Collins Ben C BC Ghosh Samik S Kitano Hiroaki H Aebersold Ruedi R Malissen Bernard B Gstaiger Matthias M

Cell reports 20170301 13

Spatiotemporal organization of protein interactions in cell signaling is a fundamental process that drives cellular functions. Given differential protein expression across tissues and developmental stages, the architecture and dynamics of signaling interaction proteomes is, likely, highly context dependent. However, current interaction information has been almost exclusively obtained from transformed cells. In this study, we applied an advanced and robust workflow combining mouse genetics and af ...[more]

PMID: 28355572

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