Identification of human membrane proteins

ABSTRACT: Typically, integral membrane and membrane associate proteins are underrepresented in proteomic experiments due to their low abundance and solubility during sample preparation. We collected two fractions upon cell lysis, one comprising the insoluble membrane fraction the other the soluble cytosolic fraction. Using detergents and additives we were able to release the proteins from the membrane fraction and subsequently perform trypsin digestion for analysis.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Hela Cell

SUBMITTER: Andreas Schmidt  

LAB HEAD: Axel Imhof

PROVIDER: PXD005063 | Pride | 2019-02-25

REPOSITORIES: Pride