N6-methyl adenosine (m6A) recruits and repels proteins to regulate mRNA homeostasis

ABSTRACT: RNA modifications are integral to regulation of RNA metabolism. One such abundant mRNA modification is m6A, which impacts various aspects of RNA metabolism including splicing, transport and degradation. Current knowledge about proteins recruited to m6A to carry out these molecular processes is still limited. Here we describe a comprehensive and systematic mass spectrometry-based screening of m6A interactors in various cell types and species. Amongst the main findings, we identified G3BP1 as a protein, which is repelled by m6A and which positively regulates mRNA stability in an m6A regulated manner. Furthermore, we identified FMR1 as a novel, RNA sequence context dependent m6A reader, thus revealing a connection between an mRNA modification and an autism spectrum disorder. Collectively, our data represents a rich resource for the community and sheds further light on the complex interplay between m6A, m6A interactors and mRNA homeostasis.

OTHER RELATED OMICS DATASETS IN: PRJNA386460

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Cell Culture, Cervix Epithelium, Embryonic Stem Cell

SUBMITTER: Rik Lindeboom  

LAB HEAD: Michiel Vermeulen

PROVIDER: PXD006498 | Pride | 2017-08-22

REPOSITORIES: Pride